Влияние фитоэкдистероидов и неробола на функциональное состояние митохондрий печени при экспериментальном сахарном диабете

Аннотация

Subjects of the inquiry: Rat liver mytochondria, phytoccdystcroids (PEC-ccdystcron, turkcstcron), ncrobol. Aim of the inquiry: Determination of Ca+2 transport, respiration and oxidative phosphorilation, ATPase activity, rotenone-insensitivity NAD.N-oxidase, rotenone-sensitive NAD.N-oxidase, succinic oxidase and cytochrome C-oxidasc, lipid contents, lipid peroxidation processes, hydrolithic activity of phospholipase A2 (PLA2) and phospholipase Al (LPLA1), liver mytochondria. Methods of investigation: polarography, pH-mctry, thin layer chromatography, spectrophotometry. The results achieved and their novelty: The administration of PES and ncrobol to the diabetic animals (DA) resulted in slowing of calcium ions, restoration of OP parameters, ATPase and oxidase activity, lipid metabolism, lipid peroxidation processes and activity of liver mytochondria lypolitic enzymes. Prolonged administration of PEC and ncrobol into the DA resulted in reduction of NAD-dependent substrates of liver mytochondria oxidation. At the same time ecdystcron and nerobol have no effect on the increase in activity of cytochrom C cuccinic oxidase. The studied preparations provided increase in phosfatidycholin; turkcstcron increases the content of (phosphatidylinosit) PI; ecdystcron and ncrobol provide partial restoration of PI content. Ncrobol provides full and PEC partial restoration of the content of posphatidylscrine. Practical value: The results presented in this work significantly broaden knowledge about molecular mechanism of PEC and ncrobol effect on the liver mytochondria under the hyperglycemia. Degree of embed and economic effectivity: The principles of correction of structure and functional disturbances in liver mytochondria membrane used in the work occupy the special place in the development of target pathogenic treatment of the patients with hyperglycemia; obtained results fulfill knowledge in rat biochemistry and allow carrying new scientific investigations and improving teaching process. Field of application: biochemistry, diabctology, physiology, pathologic physiology, hepatology, clinical pharmacology.