Technology and Qualitative Analysis of Liquid Extract Obtained on The Basis of Plant Raw Materials Polygonum Hydropiper L. And Hypericum Perforatum L.

Аннотация

This research work is devoted to the study of the technology and qualitative analysis of obtaining liquid extract from Polygonum hydropiper and Hypéricum perforátum plant raw materials. Percolation was used in liquid extract extraction. Ethyl alcohol of different concentrations was used as an extract. When conducting quality analysis, a spectrophotometric method was used. Keywords: Liquid extract, flavonoids, Polygonum hydropiper and Hypéricum perforátum, percolation, spectrophotometry. In medicine, it is used in the treatment of Introduction: Drug preparations with a twisting effect, gastrointestinal (colitis, diarrhea), oral diseases obtained from plant raw materials, are now widely (gingivitis and stomatitis) and burns of II and III degrees, used. Agents with a twisting effect are among the drugs as well as for rinsing the mouth. The plant surface of that have a local anti-inflammatory effect. They are the plant has a bactericidal effect [1,4]. used in inflammatory processes of the skin and mucous membranes [1]. Therefore, when we carried out our The purpose of the work. Obtaining liquid extract from research work, we selected Herba Polygoni Polygonum hydropiper and Hypéricum perforátum hydropiperis and Hypéricum perforátum from plant plant raw materials and conducting its qualitative raw materials with a twisting effect by chemical analysis is the goal of our research work. composition. The Polygonum hydropiper has been METHODS used since ancient times as a medicinal plant, with When obtaining a liquid extract, on the which the Greeks and Romans, al-Khimik, used it. recommendation of pharmacologists from the Polygonum hydropiper contains glycosides, vitamins C, following selected plant raw materials, herba Polygoni K, carotene, flavanoids, sugars, organic acids, hydropiperis and herba Hypérici perforátum in the astringent bitter compounds and other substances. In same proportion were pulled out and ground at a level folk medicine, tincture is used in malaria, diarrhea, of 3-5 mm spruce. Two different capacities of ethyl hemorrhoids and as a bleeding suppressor and alcohol were selected as an extract (40% and 70%). The painkiller and as a medicine for various wounds [5]. The percolation method was used in obtaining liquid Hypéricum perforátum product contains 10-12.8 extract [2,3]. Liquid extracts were studied on the basis percent additives,0.1-0.4 percent anthracene unums, of methods given in their appearance, dry residue, flavonoids (hyperoside, rutin, quercitrin, isocversitrin, alcohol concentration, density, heavy metal content quercetin, myricetin, etc.), 0.1 - 03 percent essential oil, O’zR DF, XI DF [4,5]. Quantitative analysis of the 55 mg percent carotene, 1151.8 mg percent vitamin C, resulting liquid extracts was analyzed in a ~4 mg percent choline, very small amounts of alkaloids, spectrophotometric way by the average amount of and up to 10 percent tar. Hypéricum perforátum flavonoids (in the calculation of the rutin standard products, on the other hand, have anti-inflammatory, substance) [2,4]. spasmolytic analgesic, antiseptic and twisting effects. American Journal Of Biomedical Science & Pharmaceutical Innovation 8 https://theusajournals.com/index.php/ajbspi American Journal of Applied Science and Technology (ISSN: 2771-2745) As standard, rutin flavanoid is used, in which the acetic acid in 70% ethyl alcohol, put in a 25 ml flask and maximum absorption corresponds to the maximum brought to the line of the measuring flask with 70% absorption of the sample being examined with the ethyl alcohol. aluminum chloride complex. Measurements of optical Working standard sample solution preparation (WSS). density were carried out on the UB-spectrophotometer Approximately 25 mg (exact drawer) of WSS rutin is "Agilent Technology-8253" (Germany), at a wavelength obtained, dried to a constant weight at 135°C, placed in of 350-450 nm, in cuvettes 10 mm thick. a 100 ml volume flask, dissolved in heated 80 ml of 96% Preparation of the probing solution. 1ml of the probing ethyl alcohol, then cooled and transported to the line liquid extract was placed in a 25ml volume measuring of the measuring flask with that 96% ethyl alcohol and flask, 5ml of 96% ethyl alcohol, 5ml of 5% aluminium mixed. chloride was released from a 70% solution of ethyl At the same time, after 30 minutes, the optical density alcohol. After 10 minutes, a solution of 2 ml of 5% acetic of 1 ml of a comparable solution (isn rutin) is measured acid in 70% ethyl alcohol was added. The volume of the using a sample of a compensatory solution (1 ml of solution was reached and mixed with 70% ethyl alcohol liquid extract, 5 ml of 96% ethyl alcohol and 2 ml of 5% to the line of the measuring flask. The optical density of acetic acid in 70% ethyl alcohol, put in a 25 ml flask and the solution obtained after 30 minutes was measured brought to the line of the measuring solution flask with in a 10 mm thick cuvette at a wavelength of 408nm on 70% ethyl alc