Evocation, Regeneration And Delineation, Of A Completely Unique Oxidase From C. Multiflorus ( White Broom )

Аннотация

The present work describes a study on the isolation, purification and a few features of a completely unique peroxidase from white Spanish broom, a tree legume very abundant within the northern half Spain and Portugal. Optimal conditions are proposed for enzyme extraction, removal of phenolic compounds and enzyme purification by consecutive hydrophobic, ion-exchange and size-exclusion chromatographies. Peroxidase from white broom was found to possess a relative molecular mass of 49 kDa. The spectrum of CMP showed a Soret band at 403 nm with a Rz factor of three .3. Substrate specificity and therefore the effect of some variables on the activity of CMP with guaiacol as cosubstrate have also been investigated.