The study of acute toxity and efficacy evaluation of bioactive compounds for constipation

Аннотация

Constipation is a prevalent gastrointestinal disorder that significantly impacts patients' quality of life. The present study aims to evaluate the acute toxicity and efficacy of selected bioactive compounds for the treatment of constipation. Acute toxicity was assessed using standardized in vivo models to determine the safety profile of the bioactive compounds. The therapeutic efficacy was evaluated through pharmacodynamic and biochemical analyses, focusing on bowel motility, stool frequency, and intestinal microbiota modulation. Preliminary findings suggest that the tested compounds exhibit favorable safety margins and potent laxative effects, potentially offering a novel approach for constipation management. Further clinical studies are required to validate their long-term safety and effectiveness. Keywords: Garcinia cambogia, senna, chitosan, green coffee, constipation, bioactive compounds, acute toxicity, subchronic toxicity,white mice. awareness of the long-term health benefits of natural Introduction: The World Health Organisation is ingredients by consumers. As demand for plant-derived currently facing a global problem with the increasing medicines grows worldwide, many countries are number of pathological conditions in the human body increasingly concentrating on producing new types of due to metabolic disorders. Bioactive substances plant-based medicines. derived from natural plants have become a popular treatment for improving metabolism in the human Senna (Cassia angustifolia) is a plant of the bean family. body. As we know, in recent years, the number of types This branched shrub, which is abundant throughout of drugs derived from bioactive substances obtained southern India, can reach a height of 1.8 m. Extracts of from plants has increased in the pharmaceutical this plant are used in folk medicine to treat some market. The primary reason for this is the widespread gastrointestinal disorders. hydroxyanthrazine International Journal of Medical Sciences And Clinical Research 46 https://theusajournals.com/index.php/ijmscr International Journal of Medical Sciences And Clinical Research (ISSN: 2771-2265) glycosides, also known as sennosids, have been • Senna – 40 mg reported to stimulate the peristalsis of the colon and • Green coffee – 20 mg change the absorption and secretion of the colon Composition of biologically active substance 3: resulting in fluid accumulation and excretion.b Researchers 2 have suggested that a normal daily dose • Garcinia Cambodia – 450 mg of senna for adults consists of two tablets containing 18 • Chitosan – 100 mg mg of sennoside per 90 mg tablet. Other studies 4 have • Senna – 55 mg shown that sennosids cause diarrhea due to changes in • Green coffee – 5 mg the intestinal tract. The laxative effect of this natural product is due to its anthraquinone glycosides. Composition of biologically active substance 4: [1,2,3,4,5] • Garcinia Cambodia – 400 mg Garcinia cambogia extract is quite promising for the • Chitosan – 100 mg correction of serum and tissue cortisol levels. It is • Senna – 70 mg known that the fruit of Garcinia cambogia includes organic acids, benzophenones, pectins, polyphenolic • Green coffee – 40 mg compounds, xanthocymol and isooxantocimol, METHODS cambonine, kamboginol, carbohydrates, molds and The study of acute toxic interaction described in the other biologically active substances; The content of literature with the general method adopted, disposable hydroxylic acid in fruits reaches 65%. Numerous studies into the stomach by sending special zond and toxic have shown that Garcinia cambogia drugs can prevent studied by determining the class was [23]. obesity, having hypolipidemic, antidiabetic, anti- Type and number of animals: 72 white sterile male mice inflammatory and antioxidant activity. [6,7,8]. weighing 19-21 g, quarantined for 14 days, were used Chitosan is a natural cationic polysaccharide obtained in the experiment. by the deacetylation of chitin [9]. The chemical reaction Preparation of aqueous solutions: 12.2% aqueous occurs as a result of the substitution of parts of the N- acetic groups in chitin, which are β- (1-4)-2-amino-2- solution of tested biologically active substances (1 capsule + 5 ml H2O) was prepared for acute toxicity deoxy-D-glycopyranose and β- (1-4)-Acetamide-2- deoxy-D-glicopyranosis [10]. Chitosan demonstrates study and determination of LD50. good biocompatibility, biodegradability, permeability, The laxative effect of biologically active substances 1, 2, low cell toxicity [ 11 ,12], anti-inflammatory, analgesic 3, 4 was studied on 24 white rats with a mass of 190- and blood-stopping action [13,14]. In addition, chitosan 210 g. exhibits antibacterial activity by interacting protonated