Topography, Anatomy, Histology and Morphometry of The Stomach in Experimental Rats Depending on Age

Аннотация

Based on our scientific research, comparative morphological and morphometric analysis of the structure of the mucous, submucosal, muscular and serous layers of the stomach wall of rats fed palm oil, comparative analysis of the density of the mucous glands, and evaluation and comparison of their morpho- functional changes under experimental conditions using laboratory analyses allow us to develop statistical analysis indicators of the stomach wall layers. Keywords: Rats, stomach, mucosa, submucosa, stomach length, stomach width, stomach weight, morpho-functional, organometry. Introduction: To measure the macroscopic dimensions of the stomach, Ivan Pavlov used the organometry method to study the anatomical dimensions of the stomach of rats. After the stomach was removed from the abdominal cavity, its length, height, width, and weight were measured [1, 2, 3, 4, 5, 6]. Organometry of organs, due to the possibility of statistical analysis, complements morphological data, increases the level of reliability, and demonstrates objectivity. This method is of great importance in assessing the concepts of the normal state and pathological changes of organs. A The American Journal of Medical Sciences and Pharmaceutical Research 25 https://www.theamericanjournals.com/index.php/tajmspr The American Journal of Medical Sciences and Pharmaceutical Research caliper was used to measure these dimensions of the The general morphology of the structural structures of stomach. The shape of the stomach was determined the stomach of experimental animals was studied by visually [8,10,11,13,15,19]. histological sections of the stomach prepared on a microtome, stained with hematoxylin eosin and Van For microscopic examination, the stomach was excised Gizon. To measure the macroscopic dimensions of the from the abdominal cavity and fixed in a 10% neutral stomach, the organometry method was used to study solution of formalin for 72 hours, then washed in the anatomical dimensions of the stomach of Ivan running water, dehydrated in increasing Pavlov's rats. After the stomach was removed from the concentrations of alcohol, and paraffin-embedded abdominal cavity, its length, height, width and weight blocks were prepared. Sections 5–8 μm thick were were measured. Organometry of organs, since it is prepared from them, and the general histological possible to perform statistical analysis, complements structure was studied with hematoxylin-eosin stain, morphological data, increases the level of reliability, and and the connective tissue fibers in the gastric stroma shows objectivity. This method is of great importance in were stained with the Van Gieson method. Histological assessing the concepts of the normal state and preparations were photographed using a CX40 model pathological changes of organs. A caliper was used to with an OD400 camera. measure these dimensions of the stomach. The shape of Research objective the stomach was determined visually. In our scientific work, we aimed to study the RESULTS AND DISCUSSION topography, anatomy, histology, and morphometry of The following data were obtained regarding the the rat stomach depending on age. structural components of the stomach wall of the white METHODS male laboratory rats under study. The gastric mucosa of For the study, the stomachs of 150 white male rats, 2 the rats in the control group is covered with a stratified groups, with a body weight of 48 grams to 220 grams, epithelium. The epithelial layer consists of three rows of of different ages: 21, 60, 90, 120, 150, 180 days, were basal, round and oval cells. taken. The experimental animals were kept under Basal cells are small, densely packed, and characterized normal laboratory diet conditions. These rats were by a central nucleus. The cells of the middle and upper divided into two groups. The control group continued rows are oval in shape, larger in size, with off-center to be fed a daily constant diet. The diet of the second nuclei. The apical part of the cells contains granules experimental group of white rats was restructured so indicating secretory properties. The cells of the proximal that it consisted of red palm oil. For 30 days, 1.7 Palm row of the covering epithelial tissue of the rat stomach oil from g/kg During daytime feeding, rats were given are covered with a cuticle. a special diet in the laboratory, in addition to oral through probe using an additional dose was administered into the stomach. Figure 1. Gastric wall layers in the 21-day control group. Staining: hematoxylin-eosin. X: 10x40. The muscularis mucosa of the stomach of white male internal longitudinal and external circular orientation, rats is composed of smooth muscle fibers with an and is composed of muscle layers. The outer serous The American Journal of Medical Sciences and Pharmaceutical Research 26 https://www.theamericanjournals.com/index.php/tajmspr The American Journal of Medical Sciences and Pharmaceutical Research membrane of the rat stomach has a m