Analysis of models for involving experimental liver poisoning in rats

Аннотация

In recent years, chronic liver diseases have become a significant cause of death and disability [2], there is a trend towards an increase in the number of patients with chronic liver disease. It is widespread mainly among people of working age [3], which is explained by the living conditions of modern humans and the presence of a large number of chemical compounds in the environment. This leads to changes in metabolic processes in the liver and its toxic damage. According to WHO data, over the past 20 years, there has been a trend towards an increase in the number of liver diseases leading to high mortality worldwide. Today, the number of patients with various hepatobiliary pathologies in the world exceeds 2 billion people. In the CIS countries alone, between 500,000 and 1 million people with various liver diseases are registered annually [4]. A sharp increase in the number of patients with chronic liver diseases was facilitated by an increase in the incidence of viral and toxic (alcoholic and drug) hepatitis, as well as a significant increase in the number of patients with obesity and diabetes mellitus. The hepatoprotective properties of drugs improve metabolism, inhibit peroxidation, activate lipid metabolism, and protect mitochondrial and microsomal enzymes from damage to varying degrees [1]. Keywords: Hepatitis, experimental hepatitis, paracetamol hepatitis, hepatitis induced by 40% ethyl alcohol, galactosamine hepatitis induction model, carbon tetrachloride (CCl4). Introduction: Today, despite the wide range of Currently, the following methods are used in the study preparations with hepatoprotective properties, the of experimental liver poisoning: demand for this group of preparations remains high. 1. Citing a model of carbon tetrachloride (CCl4) Scientific research aimed at solving this problem is poisoning in the liver based on the correct choice of the hepatitis model and Calculating a model of liver poisoning using carbon the correct conduct of the experiment. Therefore, we tetrachloride (CCl4) is a widely used method in began our research with the analysis of models of toxicological and biomedical research. When creating experimental hepatitis induction. American Journal Of Biomedical Science & Pharmaceutical Innovation 5 https://theusajournals.com/index.php/ajbspi American Journal of Applied Science and Technology (ISSN: 2771-2745) this model, the following steps are carried out: model is widely used for the study of liver diseases and the testing of new treatment methods. Below is the Experiment design process of creating a model of liver poisoning using For this purpose, groups are created for white male paracetamol: laboratory rats. At least 4 groups are taken, with no less Experiment design than 6 white male laboratory rats in each. The 1st group is the "intact" group, in which the disease is not caused. For this, 180-220 g of laboratory rats (for example, The 2nd group is the "control" group, where carbon Sprague-Dawley or Wistar rats) are selected and tetrachloride (CCl4) causes liver poisoning and is not grouped. For this, at least 4 groups of white male treated. The 3rd group is the experimental group, laboratory rats are taken, with no less than 6 in each. where carbon tetrachloride (CCl4) causes poisoning in The 1st group is the "intact" group, in which the disease the liver, and before this, the studied drug was is not caused. administered in a certain dose. The 4th group is the The 2nd group is the "control" group, where comparison group, where carbon tetrachloride (CCl4) paracetamol causes liver poisoning and is not treated. causes poisoning in the liver, and before this, the drug, The 3rd group was the experimental group, where which is widely used in medical practice today, is paracetamol induced liver poisoning, and the studied administered in a certain dose. drug was previously administered in a specific dose. Experiment The 4th group is the comparison group, where paracetamol causes liver poisoning, and before this, These groups were cut 0.5 cm from the tip of the tail of the drug, which is widely used in medical practice all laboratory rats, blood was taken to obtain today, is administered in a certain dose. preliminary biochemical results, and a preliminary analysis was conducted. After this, the "Intakt" group Experiment was given distilled water for 6 days, corresponding to Before starting the experiments, the rats intended for body weight. The control group was also given distilled the experiment are weighed and grouped. Blood was water for 6 days. The experimental group was given the taken from all groups of rats and the initial biochemical studied drug at 1 mg/kg or at a certain dose for 6 days. blood parameters were determined. The comparison group is given a drug that has been Modeling of liver damage using paracetamol was used in medical practice for 6 days. On the 7th day, all carried out as follows: laboratory white male rats groups, except the intact group, were adm