Determining the concentration of increasing substances and glycosides in the composition of ordinary igra

Аннотация

The article presents data on determining the content of glycosides and tannins in the plant Acorus calamus L. The content of tannins by the permanganate titration method was 1.19%, while the content of glycosides determined by HPLC was 0.0576%. The obtained results confirm the pharmacological significance of licorice root crops and the prospects for their further study. Keywords: Acorus calamus, glycoside, tannins, permanganate titration, high-performance liquid chromatography, extraction, enzymatic hydrolysis, spectrophotometric analysis, ultrasonic extraction. dried at 40-50 °C, and the content of bitter glycosides Introduction: Common acorns (Acorus calamus) are and tannins was determined. widely used in folk medicine due to their anti- inflammatory, antiseptic, and antipyretic properties. Determining the content of bitter glycosides. We can also find it in the recommendations of the great The determination of bitter glycosides was performed scholar Abu Ali ibn Sina. The rhizome of the plant on HPLC. The experiment was conducted on a HPLC contains biologically active substances belonging to model from Agilent Technologies 1200 (USA). Degager different groups, including essential oils, flavonoids, G1379A spectrophotometric detector. During the tannins, and glycosides, which determine the experiment, Agilent S with a particle size of 5 μm was pharmacological activity of the plant. The aim of this conducted on a column of 5 μm (3*150 mm). The study is to determine the content of tannins and detection was performed at a wavelength of 254 nm. glycosides in the roots of local fir. The mobile phase is 1.0% acetic acid (A) and METHODS acetonitrile (B). The flow rate is 1 ml/min, the sample volume is 10 ml. Temperature 400 °C, analysis 25 The research was conducted in accordance with the minutes. requirements of the pharmacopoeia of acorns grown in an environmentally friendly area, prepared in July- Determining the composition of tannins August. First, the raw material was cleaned of foreign A permanganatometric titration method was used to matter, washed with water, ground to 1-3 mm and determine the composition of tannins considering the American Journal Of Biomedical Science & Pharmaceutical Innovation 23 https://theusajournals.com/index.php/ajbspi American Journal of Applied Science and Technology (ISSN: 2771-2745) ability of the tannins to oxidize with a potassium completed, 5 ml of 10% trichloroacetic acid solution permanganate solution. A weight of 1.0000 g of roots was added to the reaction mixture to remove of common acorns, pre-ground to a fraction 1-2 mm in enzymatic proteins, which contributed to the size, was weighed. The raw material was placed in a precipitation of protein components. The suspension flask with a capacity of 250 ml and 50 ml of distilled was centrifuged at a rate of 3000 rpm for 10 minutes, water was added. after which the liquid above the precipitate was used for spectrophotometric analysis. The resulting mixture was heated in a water bath for 30 minutes at a temperature of 60-70°C with periodic To determine the amount of glycosides, 2 ml of ortho- stirring to ensure the extraction of tannins. After dihydroxybenzoic acid, acting as a reagent for phenolic extraction, the mixture was cooled to room compounds, was added to 2 ml of the obtained temperature and filtered through paper filters to supernatant. The volume was increased to 10 ml of remove mechanical impurities. distilled water. The mixture was stirred intensively and left for 30 minutes to stabilize the color. Quantitative analysis of the filtered extract was conducted. To create the acidic environment necessary The optical density of the solution was measured on a for the reaction, 10 ml of extract was taken and 5 ml of spectrophotometer at a wavelength of 410 nm. The 10% sulfuric acid solution was added to the titration concentration of glycosides was determined using a flask. The extract was titrated with 0.1 N potassium calibration curve constructed using a standard solution permanganate solution until a stable pink color was of rutin in the concentration range of 10-100 μg/ml. obtained and stored for 30 seconds. The amount of The following formula was used to calculate the potassium permanganate spent on titration was concentration of glycosides in the raw material: recorded. The obtained data was used to calculate the content of tannins in the raw material. The following 𝐶·𝑉 𝑡𝑜𝑡𝑎𝑙 G= · 100 formula was used for the calculation: 𝑚 𝑉·𝑁·𝐾·𝑀 where (C) is the concentration of glycosides in the T= · 100 𝑚 extract (mg/ml); (V) - total volume of the extract (ml); (m) - raw material mass (g). Calculations have shown where (V) is the volume of potassium permanganate that the content of glycosides in the roots of the solution used for titration (ml); (N) - normal potassium common acorns is 1.4%. permanganate solution (0.1 n); (K) - coefficient of Results and discussion recalculation for tannins (0.004157); (M) - the Permanganatome