Toxicological Assessment of Naja naja Venom: DNA Damage in Albino Rats

Аннотация

This study investigates the toxicological effects of Naja naja venom on DNA integrity in albino rats, with a focus on understanding its genotoxic potential. The venom of the Indian cobra (Naja naja) contains a complex mixture of proteins, enzymes, and toxins that may cause cellular damage, including DNA fragmentation. In this experiment, albino rats were administered varying doses of Naja naja venom, and the resultant DNA damage was assessed using the comet assay, a sensitive technique for detecting DNA strand breaks. Additionally, histopathological analysis of major organs was conducted to evaluate the venom's overall toxic effects. The results demonstrated significant DNA damage in the peripheral blood cells of the rats, with increased comet tail length and DNA fragmentation proportional to the venom dose. Histopathological findings showed cellular degeneration and necrosis in key organs, including the liver and kidneys. This study highlights the genotoxic effects of Naja naja venom, suggesting its potential as a hazard to DNA integrity. The findings underline the need for further research on the molecular mechanisms underlying venom-induced DNA damage and its implications for human health and safety.