Chemical analysis of polyphenols in camellia sinensis, their health benefits, and possibility for application in eye diseases

Аннотация

This article presents the results of a study on the of the creative commons attributes 4.0 License. determination of polyphenols in Black tea by high- performance liquid chromatography (HPLC). Polyphenols are bioactive compounds in plants, whose antioxidant properties have a therapeutic effect against various diseases. During the study, the main polyphenols (e.g. flavonoids and phenolic acids) in Black tea were identified and their quantitative indicators were determined. The effective effect of these compounds on eye diseases, in particular, age-related macular degeneration, glaucoma, and dry eye syndrome, was studied. The results showed that polyphenols in Karachay play an important role in protecting eye tissues from oxidative stress and reducing inflammatory processes. The results of this study serve to increase the pharmacological value of Karachay and expand the possibilities of its application in the healthcare sector. Keywords: Polyphenol, phytochemicals, Camellia sinensis, Karachoy. Introduction: Black tea (Camellia sinensis) contains many biologically active compounds, in particular The American Journal of Medical Sciences and Pharmaceutical Research 78 https://www.theamericanjournals.com/index.php/tajmspr The American Journal of Medical Sciences and Pharmaceutical Research polyphenols, which are of great importance for human black tea bags to the eyes reduces swelling and redness. health. Polyphenols have antioxidant, anti- This is especially useful for tired or sleep-deprived eyes. inflammatory, and cardioprotective properties and A cooled infusion of black tea reduces redness in the play an important role in the prevention and treatment eyes and soothes the eyes [6]. It can also be used in case of various diseases [1]. This study aimed to determine of allergies or irritation. Black tea bags or compresses the amount of polyphenols in black tea and their stimulate blood circulation, which has a positive effect health benefits using high-performance liquid on the skin around the eyes. The light hydrating chromatography (HPLC). properties of black tea help moisturize the eyes. Washing or compressing with tea leaves reduces eye Black tea is translated from various languages as red dryness. tea - black tea, which has a more diverse variety and a stronger taste than other teas. The name of the drink, Experience part which first appeared in China, is hong cha ( "red tea") Reagents and equipment used. Gallic acid was obtained due to the color of the leaves that oxidize when from Macklin (China), Salicylic acid from Rhydburg properly processed. Today, it is widely consumed and Pharmaceuticals (Germany), quercetin, apigenin, and harvested, including in China, Japan, Korea, and kaempferols from Regal (China), and rutin were isolated Singapore. While green tea usually loses its flavor from natural sources by extraction and column within a year, black tea retains its flavor for several chromatography. Water, acetonitrile, acetic acid of the years [2]. chemically pure brand, and sodium hydroxide were A study by Chinese scientists at National Chiayi used as reagents of HPLC purity. University, led by Chih-Yu Lo and Min-Hsiung Pan, The content of polyphenols in the plant was determined investigated the nutritional value of tea, mainly its using an LC-40 Nexera Lite high-performance liquid polyphenols and antioxidant properties. A meta- chromatograph manufactured by Shimadzu, Japan. analysis of observational studies concluded that black Preparation of standard solutions. Gallic acid (5.2 mg), tea consumption does not affect the development of salicylic acid (5.2 mg), rutin (5 mg), quercetin (5 mg), oral cancer, Asian esophageal or prostate cancer, or apigenin (5 mg), kaempferol (5 mg) were dissolved in lung cancer in Asian or Caucasian populations. In 96% ethanol for 20 minutes in an ultrasonic bath and addition, black tea contains polyphenols - antioxidants transferred to a 50 ml flask and made up to the mark that help maintain the balance of intestinal microflora with ethanol. 200 μl of each solution was taken, mixed, and eliminate pathogenic bacteria in the body [3]. and diluted to prepare a total of 4 different solutions. Cleans the mouth According to scientific research, the Each solution was poured into a vial and used for polyphenols in black tea protect against oral diseases, analysis. rashes, and stomatitis. Benefits for the heart European Preparation of plant extract. For the extraction of scientists have proven that those who drink 3-4 cups of phenolic compounds, 1 g of the test sample was tea a day have a 21% lower risk of heart disease. True, weighed with an accuracy of 0.01 g on an NV222 balance it is not recommended to drink black tea in a bitter manufactured by OHAUS (USA), placed in a 50 ml conical form. But if it is consumed in moderation, there is no flask, and 25 ml of 96% ethanol was added. The mixture need to worry [4]. Cancer prevention According to was extracted in an ultrasonic bath of t