DETERMINATION OF THE SPECIFIC ACTIVITY OF “VAIDAZ” GEL

Аннотация

The study investigated the specific activity and antimicrobial properties of “Vaidaz” gel - 15% (Sample 01, Shelf Life 3 years), developed at the Department of Pharmaceutical Production Organization and Quality Management of Medicines at Tashkent Pharmaceutical Institute. The research demonstrated that the drug possesses anti- inflammatory and minor antibacterial effects. The research results are documented in a scientific report on research work in accordance with the requirements of GOST and O’zDSt 2762:2018 “Good Laboratory Practice,” Tashkent 2018. Keywords: Specific activity, gel, “Vaidaz”. Relevance of the problem: The problem of drug provision, rational and safe pharmacotherapy has become one of the most pressing tasks for the activities of clinicians and pharmacists. This is due, on the one hand, to the constantly growing drug market and the volume of scientific information, and on the other hand, to the need to determine the most sought-after groups and classes of drugs. In the context of existing healthcare resources, rational use of high-quality, effective, safe drugs and the creation of new drugs by combining previously known drugs with an innovative approach is a priority task for pharmacy. Objective of the study: Determination of the specific activity of “Vaidaz” gel. Materials and methods of the study: “Vaidaz” drug - 15% gel, biological and microbiological methods. Objective of the study: To study the specific activity of the “Vaidaz” drug - 10% gel (Sample 01, Shelf Life 3 years), developed at the Department of Pharmaceutical Production Organization and Quality Management of Medicines at Tashkent Pharmaceutical Institute in an experiment on white rats. Results and Discussion: The anti-inflammatory effect of the gel was studied using the “histamine edema” method on 18 white rats weighing 180-200 g of both sexes, compared with the “Fastum gel” drug produced by A. Menarini Manufacturing Logistics and Services S.r.L, Italy. The paw volume of the rats was measured in the norm beforehand. The compared drugs were applied once topically in a thin layer 1 time a day before histamine administration and every hour during the experiment.[4] For the experiment, the rats were divided into groups of 6 individuals each. The drugs were administered as follows: 1st group (control) - 0.1 ml of 1% histamine solution; 2nd group (experimental) - “Vaidaz” drug in a thin layer + 0.1 ml of 1% histamine solution. 3rd group (experimental) - “Fastum gel” drug produced by A. Menarini Manufacturing Logistics and Services S.r.L, Italy in a thin layer + 0.1 ml of 1% histamine solution. An acute inflammatory reaction (edema) was reproduced by subplantar (between the 1st and 2nd toes of the left hind paw) administration of 0.1 ml of 1% histamine solution. The severity of the inflammatory reaction was assessed 1, 2, and 3 hours after the induction of inflammation by measuring the paw volume using a plethysmograph. 14 ` The anti-inflammatory effect (AE) was calculated using the following formula: AE = (Vexp - Vcont / Vcont) x 100, where Vexp – edema volume in the experimental group; Vcont – edema volume in the control group. The results were processed using the method of variation statistics.[2] The results obtained when studying the anti-inflammatory effect of the compared drugs showed that in rats of the control group, the paw volume after dextran solution administration increased by 62.8% after 1 hour, by 59.5% after 2 hours, and by 48.8% after 3 hours compared to the initial paw volume. With the prophylactic use of “Vaidaz” gel, the paw volume after drug administration was 25.8% less after 1 hour, 33.6% less after 2 hours, and 30.5% less after 3 hours compared to the control indicators. The AE in rats was 53.5-75.2%. Similar data was obtained when studying the anti-inflammatory activity of the “Fastum gel” drug produced by A. Menarini Manufacturing Logistics and Services S.r.L, Italy. With drug administration, the paw volume after drug administration was 16.2% less after 1 hour, 30.5% less after 2 hours, and 30% less after 3 hours compared to the control indicators. The AE in rats was 24.8-68.5%. Thus, the obtained results show that “Vaidaz” drug, developed at the Department of Pharmaceutical Production Organization and Quality Management of Medicines at Tashkent Pharmaceutical Institute, possesses anti-inflammatory activity and is not inferior in activity to the “Fastum gel” drug in an experimental aseptic arthritis in rats. The results of the experiment are presented in Table 1. Table 1. Study of the anti-inflammatory effect of the drug “Vaidaz” Increase in paw weight, ml Anti-inflammatory effect in % Group 2 h Baseline 1 hour 3 hour our Control 1,21±0,05 1,97±0,13ˣ 1,93±0,13ˣ 1,80±0,11ˣ 1,46±0,08ˣ 1,28±0,06ˣ 1,10±0,04 1,25±0,05ˣˠ “Vaidaz” (drug) ˠ ˠ 75,2% 53,5% 75,1% ““Fastum gel” (drug)” 1,34±0,03ˣ 1,08±0,04 1,65±0,05ˣ 1,26±0,02ˣˠ ˠ 24,8% 68,5% 63,0% Note: ˣ - significance of differences compared to initial values at