DETERMINATION OF THE AMOUNT OF POLYPHENOLS IN THE FOOD SUPPLEMENT "ASBOSIM" USED IN THE TREATMENT OF HYPERTENSION DISEASE

Аннотация

INTRODUCTION and mint, which has the characteristics of effective Taking into account the above, we developed a new treatment of hypertension and contains natural biologically active food supplement called biologically active compounds. This food "Asbosim" based on plants such as saffron, ajwain, supplement was approved by the Ministry of https://www.theamericanjournals.com/index.php/tajmspr 7 THE USA JOURNALS THE AMERICAN JOURNAL OF MEDICAL SCIENCES AND PHARMACEUTICAL RESEARCH (ISSN – 2689-1026) VOLUME 06 ISSUE11 Health of the Republic of Uzbekistan and put into analysis [6-10]. practice. This article presents the results of the Preparation of sample extract. For the extraction of research on determining the amount of flavonoids, 1 g of the test sample was weighed with polyphenols in the natural biologically active food an accuracy of 0.01 g on a scale, placed in a 50 ml supplement "Asbosim" [1-5]. conical flask, and 25 ml of 96% ethanol was added. The amount of water-soluble polyphenols in the The mixture was extracted in an ultrasonic bath at plant was measured using an LC-40 Nexera Lite 60 ℃ for 20 minutes. Then the mixture was cooled, high-performance liquid chromatograph filtered and made up to 25 ml with ethanol in a manufactured by Shimadzu, Japan [6-8]. volumetric flask. 1.5 ml of the extract was filtered through a 0.45 μm syringe filter placed in a vial and Preparation of standard solutions. Solutions of used for analysis [10-17]. gallic acid, rutin, and quercetin (100 mg/l) in the amount of 5 mg of each polyphenol were dissolved Determination of polyphenols. Standard solution, in methanol for 20 minutes in an ultrasonic bath sample extract Shim pack GIST C18 (150 × 4.6 mm; and transferred to a 50 ml flask and brought to the 5 μm, Shimadzu, Japan) reversed-phase column line with methanol. Then, 2.5 ml of the solution and isocritical (65:35) mobile phase was applied. was transferred to a 25 ml volumetric flask and The injection volume was 10 μL, the flow rate was brought to the flask line with 30% 0.25% aqueous 0.7 mL/min, and the column thermostat was set to solution of acetic acid and 70% acetonitrile. 1.5 ml room temperature. The analytical signal (peak of the resulting solution was filtered using a 0.45 area) of polyphenols was recorded at 254 nm μm syringe filter, poured into a vial and used for (Figure 1). Figure 1. The chromatogram at 254 nm of rutin, gallic acid, and quercetin standards. Determination of polyphenols in food in the extract of Asbosim FS. A chromatogram of the sample extract was obtained (Figure 2) and the results were processed and presented in Table 1. https://www.theamericanjournals.com/index.php/tajmspr 8 THE USA JOURNALS THE AMERICAN JOURNAL OF MEDICAL SCIENCES AND PHARMACEUTICAL RESEARCH (ISSN – 2689-1026) VOLUME 06 ISSUE11 Figure-2. The chromatogram of determination of polyphenols in the sample extract. Table 1. Amount and retention times of polyphenols in extracts. The amount of Retention time, Concentration, Vitamin sample extract sec mg/l in 100 g, mg Rutin 2,016 35,79 894,75 Gallic acid - 0 0 Quercetin 2,772 16,365 409,125 rutin and quercetin, which shows high biological From Table 1 above, it can be seen that the amount activity in the treatment and prevention of of rutin (894.75 mg/100g) in the food supplement hypertension. "Asbosim" is significantly higher than others. Also, it is known from the above table that the amount of CONCLUSION quercetin in "Asbosim" is significantly high and In conclusion, the natural food supplement that there is no gallic acid. "Asbosim" that we developed based on medicinal It is known that polyphenols such as rutin and plants contains rutin and quercetin polyphenols. quercetin normalize metabolism in the body and Among them, the amount of rutin is significantly are one of the means of treating blood pressure. higher than others, which gives this food Studies conducted in this regard have shown that supplement the property of treating and the effect of rutin and quercetin in normalizing preventing hypertension. Taking into account the systolic and diastolic blood pressure is high. This is above scientific results, people suffering from a great scientific reason to say that "Asbosim" hypertension are recommended to use the natural food additive contains a large amount of biologically active food supplement "Asbosim" https://www.theamericanjournals.com/index.php/tajmspr 9 THE USA JOURNALS THE AMERICAN JOURNAL OF MEDICAL SCIENCES AND PHARMACEUTICAL RESEARCH (ISSN – 2689-1026) VOLUME 06 ISSUE11 instead of synthetic drugs. https://www.webofscience.com/wos/auth or/record/T-7751-2017.