Структурнофункциональная организация и закономерности экспрессии гена тиреоглобулина при опухолеобразовании в клетках щитовидной железы

Аннотация

Subjects of research: samples DNA received from blood of sick and healthy donors, the thyroid glands of patients removed during operation concerning various tumors. Purpose of work: studying structurally - functional features and laws expression TG gene at various tumors of a thyroid gland, studying of the molecular-genetic mechanism of formation of tumors of a thyroid gland, studying of infringements of regulation expression TG gene and creation of theoretical base for early diagnostics of these diseases. Methods of researches: allocation of polyribosomes by a magnesium method, allocation total RNA, reception of immunosorbent PABC-TG:aTG, allocation high-molecular DNA, processing DNA restriction endonucleases, electrophoresis products of hydrolysis DNA by restriction endonucleases, hybridization of the nylon filters " Biodan " containing fragments DNA with 32P- marked cDNA, polymerase chain reaction. The results obtained and their novelty: it is received immunosorbent PABC-TG:aTG. The size of a population individual TG polyribosomes is determined Is shown, that at papillary adcnocarcinomc decrease of the maintenance individual TG polyribosomes is observed. At papillary adcnocarcinomc chromosomal aberrations that leads to genetic instability arc revealed, frequency of chromosomal aberrations makes 2,8 %. It is shown, that at papillary adcnocarcinomc occur infringement of regulation expression TG gene at a level of synthesis mRNA, i.e. at a level of a transcription. Practical value: Introduction in practice of molecular-genetic methods of researches of tumors of a thyroid gland stimulate more full understanding of the mechanism canccrogcnesis, that is essential criterion of the forecast of an outcome of disease. Degree of embed and economic effectivity: Results of researches can be used by development of approaches for early diagnostics of tumors of a thyroid gland, and also at reading rates of molecular biology and biochemistry of hormones at biological faculties of Universities, and rates endocrinology in medical institutes. Field of application: biochemistry, molecular biology, biotechnology, medicine, endocrinology.