Выделение, очистка и свойства глюкозоксидазы, выделенной из Aspergillus Niger

Аннотация

Subject of enquiry: different types of mycoses. Aim of enquiry: develop methods of allocation, clearing glucosooxydasa from Aspergillus Niger, investigate of properties and compare of this method with another Methods of enquiry: methods of cultivation, deferential centrifigure, chromatography, gel filtration, immunologic investigation. The results achieved and their novelty: For the first time in Uzbekistan was allocated glucosooxydasa from Aspergillus Niger and properties were investigated. Effectively implementation of this ferment in quantity in biologic fluid was determine. Practical value: antibody for glucosooxydasa were get and created affine column, which let for ones allocate this ferment. On the basis of allocated ferments was build set for determine of glucose in biological fluids. Degree of embed and economic effectivity: obtained results expand available representations about allocation, clearing and properties ferments; will help to create domestic sets for definition glucose in biological fluids. Sphere of usage: Biochemistry, medicine, laboratory diagnostics.