Ингибиторы эстераз яблонной плодожорки (Laspeyresia Pomonella L.) и кукурузной совки (Pyrausta Nubilalis Hb.) и изучение их синергетических свойств

Аннотация

Object of the research: pest insects of Laspeyresia pomonella I., and Pyrausta Nubilalis Hb. The purpose of work: study of cholinesterase and carboxylesterase ferments of the pest insects of Laspeyresia pomonella L. and Pyrausta Nubilalis Hb. on the basis of subtract - inhibitors methods as well as synergist properties influencing on their phosphororganic inhibitors. Methods of research: in the process of research, preparation of homogenates from cells of insects, substrate-inhibitors in studying of the activeness and properties of ferments, electrophoreses, calorimetric and Kamovsky methods as well as toxicological and synergetic methods in studying of inhibitors were used. The obtained results and their novelty: for the first time it has been researched cholinesterase and carboxylesterase ferments of the pest insects of Laspeyresia pomonella L. and Pyrausta Nubilalis Hb. adapted to natural and pesticides and the activeness dynamics in their ontogeny development. It was established that the activeness of the best adapted populations of carboxylesterase ferments to the pesticides, carboxylesterase is produced against the activeness of the used pesticides in the organisms of insects. Moreover, on the basis of studying their properties and analyzing information of the used literature, it was established that the activeness of cholinesterase and carboxylesterase ferments in the organisms of the selected insects, these ferments exist in the cells of their bodies and their activeness degree variables according to the types of insects and plants, and the conditions where their organs meet. The practical value: the carried out researches serve as a basis for working out scientifically proved aids on creation physiological active matters against selected insects and ruling metabolic activeness of ferments. Degree of embed and economic efficiency: the given pesticides can be used as synergist against established inhibitors influencing highly on the activeness of carboxylesterase ferments of selected insects. Moreover, the achieved results of the dissertation can be used as a teaching material on biochemistry, nature defense and entomology as well as on special courses of enzymology in higher education institutions. Field of application: bioorganic chemistry, ecology, entomology and agriculture.