Особенности ультраструктурных изменений в роговице кроликов при ее ожогах щелочью и коррекцией энтеросорбентом полисорб

Аннотация

30 rabbits took part at the experiment. Cornea of some of them was burned with alkali; conjunctival sac of the others was washed with the “Polisorb” sorbent solution after the combustion. Gross destructive chang-es in cornea arose already after the combustion and progressed till the end of the experiment. An hour after the combustion in the setting of homogenized tissue of the cornea frontal epithelium we also found cells with signs of gross destructive damages in its central parts. Cornea proper substance was destructed as well. De-fibring and fragmentation of collagen fibrillas was also discovered, their parallel orientation was getting lost. Changes in the group of animals, which were treated with “Polisorb”, were less significant than in the group of animals without correction. In an hour after the combustion the destructive changes in the frontal epitheli-um were almost the same as in the group of animals whose conjunctiva was not treated with “Polisorb” sorbent. Though, we discovered cells with partially residual structural elements more often. Destructive changes in the cornea proper substance were moderate. In the group of animals without correction destruc-tive changes in all layers of cornea progressed even later, their maximum development occurred in 24 hours after the combustion. In as little as 12 hours after the investigation start not only epithelial cells of the injured part of the cornea, but also its marginal area incurred obvious destruction. Intercellular spaces were exagger-ated and vacuolated. The proper substance was completely disorganized. In the contrast, in 12 hours after the combustion dehydration processes began to take off in the cornea of the animals whose conjunctive sac was washed with “Polisorb” sorbent. When morphological pattern at the combustion area was steady, we discov- ered residual and functionally active epithelial cells with moderately destructive changes in the marginal ar-ea. The eye structure was regenerating during 24 hours of the investigation