DEVELOPMENT THE THORACIC CAGE IN THE EARLY STAGES OF THE POSTNATAL PERIOD THE INFLUENCE OF ANTENATAL DIABETES

Аннотация

The material of the study was 32 mature rats and their offspring. We used informative methods such as histological and histochemical, morphometric. As a result of the study, morphological changes in the bone-cartilaginous- ligamentous structure of the rib-sternal complex in the offspring obtained from pregnant rats with diabetes mellitus in the period of early ontogenesis are presented. It has been established that the negative impact of antenatal diabetes mellitus on the development of the connective tissue formation of the sterno-costal complex. This article describes the morphological changes in the anatomical structures of the rib-sternal complex in rat pups born from pregnant individuals with experimental diabetes mellitus. KEYWORDS Cartilage, rib-sternal complex, experimental alloxan diabetes, alloxan, chondrocytes, rats. Volume 02 Issue 04-2022 23 International Journal of Medical Sciences And Clinical Research (ISSN – 2771-2265) VOLUME 02 ISSUE 04 Pages: 23-29 SJIF IMPACT FACTOR (2022: 5. 705) OCLC – 1121105677 METADATA IF – 5.654 Publisher: Oscar Publishing Services Servi INTRODUCTION The study was performed on 32 white laboratory rats weighing 150-200 g. The animals were kept in Diabetes mellitus is a global problem, and although it vivariums with food and water according to a is receiving more and more attention, its importance standard ration. The animals were divided into two is growing year by year. The number of patients is groups for the study. The control group produced 10 growing rapidly. While the total number of people rats and the male rats were in a 3: 1 ratio and were with diabetes in the world has increased fivefold since injected with 0.5 ml of 0.9% sodium chloride solution 1980, in 2018, 422 million people suffered from the once. The study group consisted of 32 rats and the disease, accounting for almost 10 percent of the male rat had a 3: 1 ratio. On the fifth day of gestation, world’s population. While maintaining the current experimental diabetes mellitus using an alloxan model situation, the number of cases is projected to double was called in rats. A mixture of alloxan 150 mg / kg and by 2030 and account for 20 percent of the world’s distilled water was administered to the experimental total population [WHO 21.10.2021]. Diabetes mellitus is group by a single intraperitoneal injection into the a chronic disease that develops when the pancreas abdominal cavity. An increase in blood glucose to does not produce enough insulin or the body is unable approximately 350 mg / dl (Plus Satellite.Russia) to use the insulin it produces effectively. Insulin is a confirms hyperglycemia. In our experimental rats, 15 hormone that regulates blood sugar level [2]. A minutes later, the rats were weaned. After 20 common consequence of uncontrolled diabetes is minutes, the tails began to turn blue. After 3 hours, hyperglycemia or high blood sugar, which over time thirst and polyuria were observed. The next day there can cause serious damage to many body systems, was less convulsions and tachycardia. In a 20- to 24- especially nerves and blood vessels. Changes in the day follow-up, the study group found that the rats shape and functional structure of the chest affects the had low mobility, weight loss, long-term wound functional state of the organs in the cavity of the cage healing, and hair loss. Of the 22 rats taken for the [2]. Lack of information on the morphofunctional experiment, 40% died. The study materials were properties of the rib –thoracic complex leads to components of the 7-14-21-30-60-day-old thorax of serious shortcomings and errors in the prevention and young rats born to mothers with experimental treatment of injuries and deformities in certain areas. diabetes. The study required histological analysis the rib-sternal complex of the experimental group. The Scientific research in this field is not only scientific but animals were decapitated under general anesthesia also of practical importance. All of the above allows us using a guillotine knife in accordance with ethical to draw conclusions about the problems developed by us and the relevance of the rib-sternal complex guidelines. For histological examination and incision was made in the rib-sternal complex (IPSUM due to the prevalence of its deformations and injuries PATHOLOGY). The samples taken for the experiment and their morphofunctional substantiation. were immersed in 10% formalin (Biovitrum). The The purpose of the work. To study the dynamics of sample was dried in growing alcohol and histologically morphological changes in the rib- sternal complex of processed (STP 120, Thermo Fisher). Then paraffin rats with experimental alloxan diabetes. was poured (Histo Star, Thermo Fisher). The incisions were made in a microtome 4–5 sm thick (HM 325). The