“HYPOSEDAF” DRY EXTRACT’S AMINO ACID AND ELEMENTAL COMPOSITION STUDY

Аннотация

Research was undertaken to examine the amino acid and elemental composition of “Hyposedaf”, a dry extract known for its hypotensive properties. Quantitative analyses of both micro and macro elements were conducted using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Additionally, the amino acid content was quantitatively analyzed through High-Performance Liquid Chromatography (HPLC). This study identified 20 different amino acids in the “Hyposedaf” dry extract, 10 of which are essential. Notable amino acids, such as histidine, cysteine, tyrosine, methionine, valine, asparagine, and leucine, amounted to a total of 29.15 mg. Of all detected amino acids, 46.5% were classified as non-essential, while 53.5% were essential. The elemental analysis of the “Hyposedaf” dry extract revealed 61 different elements, including 7 essential elements (Co, Cr, Cu, Fe, Mn, Se, Zn) and 4 conditionally necessary elements. Key elements like potassium, calcium, sodium, zinc, iron, magnesium, and phosphorus, found in substantial amounts, are crucial for human health. The concentrations of heavy metals and arsenic in the dry extract were within safe limits according to the Sanitary-epidemiological rules and norms, complying with the quantitative standards State Pharmacopoeia of the Republic of Uzbekistan (Volume 1, Issue 1) and State Pharmacopoeia of the Russian Federation XIV (RF) [1,2,3]. The findings indicate that “Hyposedaf” dry extract possesses significant value, offering potential for medical application and the development of other medicinal forms. Volume 04 Issue 01-2024 102 American Journal Of Biomedical Science & Pharmaceutical Innovation (ISSN – 2771-2753) VOLUME 04 ISSUE 01 PAGES: 102-112 SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) OCLC – 1121105677 Publisher: Oscar Publishing Services Servi KEYWORDS Dry extract, micro and macro elements, amino acids, HPLC method, mass spectrometry.