СОВЕРШЕНСТВОВАНИЕ ТЕХНОЛОГИИ ПОЛУЧЕНИЯ ХЛЕБОПЕКАРНЫХ ДРОЖЖЕЙ С ИСПОЛЬЗОВАНИЕМ КЛУБНЕЙ ТОПИНАМБУРА (Helianthus tuberosus L.)

Аннотация

Subjects of research: In this object of study used an extracts from the topinambour tubers, sweet potato and collection culture of baker's yeast of Saccharomyces cerevisiae. Purpose of work: Screening for available raw materials - substitutes for molasses from non-traditional substrates and the selection of high-level, productive strains for the production of baker's yeast with high enzymatic activity and generative power. Methods of research: Identification of carbohydrates, protein, inulin, sucrose, phosphorus, starch, thin-layer chromatography, cultivation and isolation of biomass, counting live and dead cells, determination of enzyme activities (inulaza, zymase, maltase, invertase), to determine the quality of bread and statistical analysis the data. The results obtained and their novelty: The regulation of inducible enzyme activity, patterns of biomass accumulation of yeast, consisting of actively growing cells with components of the nutrient medium containing polifruktozan, obtaining enzymatically active yeast biomass with a wide spectrum of effect. Create of a preparation of high-level, competitive commercial baker's yeast, using as a nutrient substrate tubers of topinambour - a potential substitute for molasses. Practical value: Developed the method for the preparation of nutrient medium for growing yeast on the basis of inulin containing raw materials, where the main source of carbon used an extract of topinambour, and the expediency of its usage. Based on theoretical principles and experimental data have an integrated part of the culture medium on the basis of inulin of topinambour extract, containing the sources of nitrogen, phosphorus, potassium, magnesium and vitamin complex. The developed technological regulations for the production of baker's yeast in a medium with a topinambour production schedules for manufacturing. Degree of embed and economic effectively: Cost-effectiveness of the use of yeast grown on inulin containing raw materials amounted to 1870 sum/kg (2009), compressed yeast, 1950 sum/kg for molasses. Practical value: Developed the method for the preparation of nutrient medium for growing yeast on the basis of inulin containing raw materials, where the main source of carbon used an extract of topinambour, and the expediency of its usage. Based on theoretical principles and experimental data have an integrated part of the culture medium on the basis of inulin of topinambour extract, containing the sources of nitrogen, phosphorus, potassium, magnesium and vitamin complex. The developed technological regulations for the production of baker's yeast in a medium with a topinambour production schedules for manufacturing. Degree of embed and economic effectively: Cost-effectiveness of the use of yeast grown on inulin containing raw materials amounted to 1870 sum/kg (2009), compressed yeast, 1950 sum/kg for molasses. Field of application: food industry, commercial yeast used in bread production.