ПРОТЕИН SP-2 ИЗ ШРОТА СОИ, ИЗУЧЕНИЕ ЕГО ПРОТИВООПУХОЛЕВОЙ АКТИВНОСТИ

Аннотация

Subjects of research: The object of study is the sp-2 protein complex from soybean cake. Purpose of work. Isolation of the sp-2 protein complex from soybean cake, the study of its physical and chemical characteristics and specific anticancer activity on AKA.TOL, B-16 melanoma and breast cancer strains. Methods of research. Column chromatography, electrophoretic studies, determination of amino acid composition and N-terminal amino acids, radiometric analysis on incorporation of 14C - thymidine, imunnchistochcmical analysis, microscopy, imunne enzyme analysis, PCR analysis. The results obtained and their novelty. For the first time sp-2 protein complex was isolated from soybean cake and its physical and chemical properties were studied. The obtained purified protein complex consists from 5 proteins with a molecular mass of the core component of 30 kDa, isoelectric point of pl -3,8; 3,9 and 8,2. Amino acid composition: Ala, Arg, Asp, Vai, His, Glu, Gly, He, Leu, Lys, Met, Pro, Ser, Tyr, Thr, Phe. N-tcrminal amino acids: threonine, glycine, serine, proline, valine. The sp-2 protein has shown high antitumor activity in vitro and in vivo. Practical value. Application was submitted to the State Patent Agency of the Republic of Uzbekistan №IAP 20080420 «The method of extraction of protein complex from plant material cake ». The results will help to increase fundamental knowledge of modem science regarding the mechanism and effects of proteins on tumor cells, allow to understand the new strategy for treating malignant tumors and to develop new anticancer drugs based on proteins extracted from soybean. Application of results. In a result of the research sp-2 protein was proposed as an antitumor agent. In a framework of applied research under the grant of FA-A11-T174 on "Development of anti-tumor protein preparation from soybean cake and isolation of extra pure enzymes from local raw materials" a drug form of the preparation is being developed and the necessary technical documentation by the Temporary Pharmacopeias paper project for submission to the Pharmacopeias Committee of the Republic of Uzbekistan. Field of application: chemistry of natural and physiologically active substances, medicine.