Влияние комплексного препарата «Этис-2» и сальмонеллеза других видов антибиотиков на водителей

Аннотация

This article provides information that the indicators of sensitivity to antibiotics in relation to aqueous and oil emulsions of various concentrations of the drug ETIS-2 to the causative agent of salmonella enteritidis were carried out in laboratory conditions. Keywords: S.Enteritidis, S.Typhimurium, S.Dublin, antibiotic, strain, GPA (meat peptone agar) and GPQ (meat peptone broth), ETIS-2 complex preparation, susceptibility, resistance, pathogen, concentration, ratio, dilution. Introduction. Currently, salmonellosis is one of the important problems not only in our republic, but also in the veterinary and medical fields worldwide. Salmonella Enteritidis, Salmonella Typhimurium and Salmonella Dublin pathogens are often found among farm animals and cause damage to livestock. It is scientifically known that these Salmonella serovars cause foodborne outbreaks in animals and humans [1]. For the prevention and treatment of salmonellosis, sulfanilamide, nitrofuran preparations and antibiotics of various groups are used. The complex drug "ETIS-2" consisting of pharmacopoeia drugs was developed by the scientists of VITI Tuberculosis Research Laboratory against diseases of bacterial etiology. If the ETIS-2 complex drug is used, there is no need to use other types of antibiotics or other treatment agents [12]. The goal of our research is to determine the sensitivity and resistance of different concentrations of antibiotics and ETIS-2 not only to salmonella species, but also to other types of bacteria. The purpose of the study is to study the effect levels of the ETIS-2 drug and some antibiotics on Salmonella Enteritidis pathogens, to compare the effectiveness of these drugs in the treatment of salmonellosis, and to analyze the hematological changes in the body of treated sheep. Methods. Salmonellosis is an acute infectious disease of young animals in septic form, characterized by fever, gastrointestinal disturbances, and diarrhea. Calves are infected with salmonellosis mainly from 3-4 weeks to 4 months, piglets to 4 months, sheep at any age, poultry in the first days of life, and mares are infected with specific pathogens (S. pullorum, S. gallinarum) in the mother's womb [5]. The causative agent belongs to the salmonella group, polymorphism is clearly expressed in the microbial cell, it is well stained with aniline dyes. Good growth in GPA and GPQ. Biochemically, it does not form indole, releases hydrogen sulfide, does not curdle milk. It remains unchanged in lactose and sucrose. Glucose, mannitol, maltose form gas and acid. The microorganism produces a strong poison (endotoxin) from itself. It shows different sensitivity and resistance to antibiotics and sulfonamides[6]. In case of disease, animals are recommended to be divided into the following groups after clinical examination and thermometry: 69 Procedia of Theoretical and Applied Sciences Volume 7 | May 2023 ISSN: 2795-5621 Available: http://procedia.online/index.php/applied/index 1) healthy; 2) a disease is suspected; 3) clearly infected; 4) animals that have recovered from illness. Each group should have separate feeders-shepherds and equipment. It will be necessary to establish nutritious and high-quality feeding. Levomycetin, syntomycin, tribrissen drugs are recommended for treatment. When a complication of pneumonia is observed, it gives a good treatment result when it is used in combination with antibiotics, sulfanilamides (norsulfazol, disulfan, etazol, sulfadine, sulfademyzin) [6]. Antitoxin or hyperimmune blood serums used against salmonellosis also give very effective therapeutic results. Always determining the sensitivity of pathogens to various drugs increases the effectiveness of treatment measures. This task is assigned to laboratories working with infectious diseases [7]. Discussion. In order to recommend antibiotics to sick animals, there should be a number of clinical signs, including the appearance of purulent sputum, changes in blood analysis, that is, an increase in the number of leukocytes and neutrophils, indicators of inflammation, an increase in the amount of C- reactive protein. Bacteriological cultures are grown from body fluids and the results are analyzed. Depending on the type of antibiotic that is effective against the infection, the causative colonies that grow in this process can be recommended one of the antibiotics. In this period, the wrong or mixed antibiotics have an adverse effect on the liver, kidneys and intestines, not on the microorganisms, and lead to various complications [8]. With the help of the complex drug "ETIS-2" composed of pharmacopoeia drugs against diseases of bacterial etiology, many farms in the republic were cured of tuberculosis and the expected economic damage was prevented, as well as the risk of the disease being transmitted to people was eliminated [11]. "ETIS-2" is a combination of the components included in the complex drug, which creates its advantage over other bacteriostatic drugs. Such a combina