TRIFOLIUM REPENS L. DIVERSITY IN NEPAL: A COMPREHENSIVE VARIETAL ASSESSMENT

Аннотация

INTRODUCTION breeding, and sustainable utilization. Trifolium repens L., commonly known as white clover, holds significant ecological, agronomic, and This study aims to address this gap by conducting economic importance globally. As a versatile a comprehensive assessment of varietal leguminous species, it contributes to soil fertility, differences in Trifolium repens across Nepal. forage production, and biodiversity conservation. Through field surveys, morphological In Nepal, where agriculture plays a central role in characterization, and molecular analyses, we seek the economy and livelihoods of rural communities, to identify and classify the diverse varieties of Trifolium repens thrives in diverse agroecological Trifolium repens present in the country. By settings, demonstrating adaptability to various elucidating the genetic diversity and population environmental conditions. structure of Trifolium repens, we aim to provide valuable insights into its evolutionary history, Despite its widespread distribution in Nepal, ecological adaptations, and potential for genetic Trifolium repens exhibits remarkable diversity in improvement. morphological characteristics, adaptation to different ecological niches, and agronomic traits. Furthermore, this assessment is vital for informing This diversity offers immense potential for conservation strategies to safeguard the genetic harnessing the species' benefits in agriculture and resources of Trifolium repens and enhance its ecosystem management. However, a resilience to environmental challenges such as comprehensive understanding of the varietal climate change and habitat degradation. differences within Trifolium repens populations in Additionally, understanding the varietal diversity Nepal is essential for effective conservation, within Trifolium repens populations can facilitate https://www.theamericanjournals.com/index.php/tajabe 8 THE USA JOURNALS THE AMERICAN JOURNAL OF AGRICULTURE AND BIOMEDICAL ENGINEERING (ISSN – 2689-1018) VOLUME 06 ISSUE05 targeted breeding efforts to develop improved genomic DNA was extracted from representative cultivars with desirable agronomic traits, thereby samples, and molecular markers such as SSRs or enhancing forage productivity and agricultural AFLPs were employed to assess genetic diversity sustainability in Nepal. and population structure. PCR amplification of target loci followed by gel electrophoresis enabled In this context, our study contributes to the the visualization and analysis of genetic variation broader objectives of biodiversity conservation, within Trifolium repens populations. The sustainable agriculture, and food security in Nepal. morphological and molecular data obtained were By exploring the diversity within Trifolium repens subjected to multivariate statistical analysis to populations, we aim to unlock the full potential of identify patterns of variation and classify Trifolium this valuable species for the benefit of both repens varieties. The integration of morphological ecosystems and communities. Through a and molecular analyses provided a comprehensive comprehensive varietal assessment, we strive to understanding of varietal differences within pave the way for informed decision-making and Trifolium repens populations in Nepal, laying the effective management of Trifolium repens groundwork for informed conservation strategies resources in Nepal and beyond. and targeted breeding efforts.