Влияние наночастиц диоксида кремния на прооксидантный и проапоптический эффекты ацетата свинца

Аннотация

The established capability of nanoparticles to enhance the transport of chemicals and drugs into cells and through the barriers of the body makes the possibility of potentiating the toxic effects of chemical contami-nants when introducing into the body together with nanoparticles an urgent matter. In neutrophils of blood by means of cytophluorimetric method, the formation of active forms of oxygen was determined using dichloroflu-orescein diacetate, the intensity of apoptosis and necrosis – by the level of cells labeled with annexin V and propidium iodide, and also the number of cells with reduced mitochondrial transmembrane potential was eval-uated. Maximal changes in all indices were verified in the group of animals administered with SiO 2 nanoparti-cles and lead acetate. In this case, the active forms of oxygen output and the level of apoptotic and necrotic al-tered blood neutrophils of animals and the number of cells with decreased mitochondrial transmembrane po-tential increased significantly in the animals administered with nanoparticles and lead acetate compared to the animals administered with the toxicant only. The conclusion has been drawn that silicone dioxide nanoparticles enhance the capability of lead acetate to increase the production of intracellular active forms of oxygen, the level of apoptotic and necrotic altered blood neutrophils, and the number of cells with decreased mitochondrial transmembrane potential