Определение иммунобиологической реакции у овец Хламидиоз

Аннотация

The article reports on the stability of the immune system in the body when using the GOA formalin vaccine against sheep chlamydiosis. Key words: Chlamydia, immunoglobulin, vaccine, immunity, immunophone, antigen, microorganism, receptor, serotype. Relevance of the research. Chlamydiosis in small and big horned animals is well documented to inflict significant economic harm to all farms in our nation. Investigations have shown that chlamydia is responsible for an average of 12 percent of abortions in animals. Up to 50% of miscarriages in farm animals are caused by chlamydia, claim I.I. Nosov and A.A. Volkova. The care of sick animals and the efforts to stop the sickness cost a lot of money. According to the literature, chlamydia prevalence rates for small horned animals were 18- 43.6 in the USA, 9-21.6 in Canada, 16-24.8 in the Netherlands, 18-57 in France, 14-46.4 in England, 6-26.2 in Australia, and 3-34 percent in Israel. Chlamydia is a contagious, enzootic infectious illness that causes inflammation of the lungs, abortion in the second part of the cervix, incompetent lambs and calves (usually in young animals), and inflammation of the placenta, especially the cotyledons. (pneumonia). Chlamydia abortus ovis, a member of the Chlamydiacea family and Chlamydiacea psittaci genus, is the responsible party. Chlamydia is a parasite that may grow up to 250–300 nm in size. They are bacteria with thick cell walls that carry DNA and RNA. The chlamydia-causing agent has a complicated antigen structure with three antigenic centers that are unique to the genus, species, and serogroup. Its gender is lipopolysaccharide because, like gram-negative bacteria, it has a thermostable cell wall. The binding epitope, which establishes the specificity of the genus and includes a unique receptor found in carbohydrates, and an oligosaccharide molecule with three monomers are the two components that define antigenicity. (G.A. Dmitriev et al., 1999). The particular location of cysteine-rich amino acids in the protein membrane of species-specific determinants varies between antigen serotypes. Purpose of the research. The key criteria of our experiment to assess the efficacy of the vaccination is stability of the impact of the GOA formalin vaccine against sheep chlamydiosis on © 2023, IJOT | Research Parks Publishing (IDEAS Lab) www.researchparks.org | Page 1 Copyright (c) 2023 Author (s). This is an open-access article distributed under the terms of Creative Commons Attribution License (CC BY).To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/ INTERNATIONAL JOURNAL ON ORANGE TECHNOLOGY https://journals.researchparks.org/index.php/IJOT e-ISSN: 2615-8140 | p-ISSN: 2615-7071 Volume: 5 Issue: 4 | April 2023 the immune system using IgM and IgG test kits created by JV "UNIGEN." Research object and methods. Research was conducted in VITI's Microbiology, Regional Diagnostics, and Young Livestock Disease Research Laboratories as well as under production circumstances at the livestock complex in the Kashkadarya region's Dehqonabad district called "Karakolchilik Shirkati named after M. Ibragimov." The quantity of immunoglobulins and how they affected the infections affected the body's ability to fight against germs. Farm animals almost never have immunoglobulin-E or immunoglobulin-D identified in them. (F.J.Bourne et al. 1978). The first step of immunological responses is marked by the appearance of IgM from macroglobulins. The primary immunoglobulin in blood serum is IgG, which comes in two varieties: Ig–G and Ig–G . The 1 2 primary cellular components of the body, in addition to immunoglobulins, are macrophages (monocytes), as well as active T and V lymphocytes, which guarantee the body's resistance against pathogens and viruses. (Green S.A., Albulov A.I., Ruban E.A., Green A.V. .). The morphological and pathological status of the body's tissues and cells is negatively impacted by the antibiotics used to treat the disease. It is important to remember that polyclonal activation syndromes are what lead to false positive outcomes in such enzymatic and sequential processes. The creation of specific defensive protein enzymes against diverse foreign antigens entering the body is simultaneously stimulated by superantigens, which are present in the individual (ontogonistic) phase of the animal body, and V cells respond in a particular manner. In actuality, these activities manifest as a simultaneous, non-specific rise in the titer of antigens to several infections. According to literature sources, technical flaws in the production of the response as well as immunodeficiency circumstances may be to blame for false negative findings in the detection of antigens. A specific apparatus (colorimeter) was used to measure the color intensity of the liquid in the tablet wells after the reaction had halted, and a special gadget was utilized to compute the results. The control samples' optical densities were compared, and the anal