Bioavailability of fluoride from 3 different toothpastes in vivo

Аннотация

batch number BN 4170T2), containing 1350 ppm of fluoride as NaF, potassium chloride and triclosan in a base of silica. The aim of the present 3-leg crossover study was to assess MFP (Signal Protection Caries-Blancheur, Unilver, Brussels, the oral bioavailability of fluoride delivered with two NaF toothpastes (one longer marketed 1350 ppm and one recently Belgium, batch number 718561WA) containing 1450 ppm of fluoride as sodium monofluorophosphate, sodium bicarbonate introduced 1450ppm F), and a MFP toothpaste (1450 ppm F). Ten in a base of calcium carbonate and silica. adult volunteers brushed their teeth for 1 min, followed by rinsing with 10 ml de-ionised purified water for 10 s. Samples of The volunteers were given a fluoride-free toothpaste about 2mL of whole mixed unstimulated saliva were collected (Sensodyne Previon Classic, GSK, Genval, Belgium, batch number 377 AT2) to be used 1 week prior to the experiment and in the 1- 1,5,10, 20, 30, 60,180 min after brushing. Individual salivary week washout periods between experiments. The morning of the fluoride-time plots were established and the area under the curve (AUG) calculated. Стах and AUC of MFP were significantly experiment, no tea and sea-food was permitted to exclude their influence on the baseline salivary fluoride concentration. (p 0.05).The newly introduced NaF toothpaste had a sufficient (Falcon, Becton-Dickinson, Franklin Lakes, USA). Thereafter, bioavailability of fluoride comparable to that of standard NaF volunteers brushed during 1 minute with 1 gram of toothpaste toothpaste. The MFP toothpaste showed a lower availability of applied on a new toothbrush. The excess toothpaste/saliva slurry ionisable fluoride. Key words: sodium fluoride, sodium monofluorophos- was expectorated in another graduated centrifuge tube, followed by a short rinse with 10 mL of purified water, which was also phate, toothpaste, saliva collected. Further salivary samples were obtained after Publication history: this work was based on the master thesis 2,5,10,20,30,60 and 180 minutes after the brushing stopped. of O. Jamcikova (2008, VUB) Fluoride in saliva was assessed after addition of 100 mL TISAB III to 900mL saliva. The solution was stirred after addition of Introduction TISAB. The toothpaste slurry and rinsing solutions were analyzed after 2-fold dilution and addition of TISAB. Fluoride was then Fluoride-containing toothpastes are thought to be the analyzed electrochemically.The electrode reason of decline of caries in many countries [Bowen, 1995]. However, erosion poses a new threat to dental hard tissues 20 ТЕРАПЕВТИЧЕСКАЯ СТОМАТОЛОГИЯ was calibrated before each series of measurements against a [Hattab, 1989] and should be assessed regularly. When we standard series of NaF (Merck, Darmstadt, Germany) in ul- compare the present data to a previous study with comparable trapurified water with 10%TISAB III added. Millivolt readings experimental conditions [Issa and Toumba, 2004], we obtained were obtained after 5 minutes eguilibration under constant comparable results. A difference could be found in the post-rinse stirring. The fluoride concentration was determined using the fluoride concentration since in the present study rinsing was "standard curve" option of the computer software Prism (Graph preceded by expectoration of toothpaste slurry. This could be Pad, USA) and corrected for dilution. The same program was seen as a "worst-case"situation, since it is reported to eliminate used to calculate the area under the fluoride-time curve (AUG) about 50% of the administered fluoride [Sjogren et al., 1994]. after subtracting the baseline values. The maximum Expectoration of toothpaste slurry after brushing was reported in concentration (Стах) was derived from the curves. about 40% of the subjects in a Brazilian study on fluoride AUG and Стах values were compared statistically using the retention [Oliveira et al., 2006]. Friedman test for related samples followed by comparisons with In the past there was much discussion about the eguiv- the Wilcoxon test with Bonferroni correction for multiple alence of sodium fluoride and sodium monofluorophosphate in comparisons. The individual fluoride concentrations were terms of bioavailability and caries preventive potential [Stookey compared to baseline with a paired Wilcoxon test. Statistical et al., 1993; Saporito et al., 2000]. It was shown that the amount significance was accepted at a p-value of < 0.05. Calculations of ionisable fluoride was lower in MFP toothpastes [Issa and were performed using the SPSS software package, version 16. Toumba, 2004]. Bruun et al. [1984] showed that the total salivary Post-hoc power was determined using g*power 3.1.9.2. fluoride, measured with gas chromatography was