INVESTIGATING THE IMPACT OF SUCROSE ON MICROBIAL PECTIN ESTERASE AND PECTATE LYASE ACTIVITY

Аннотация

Pectin esterases and pectate lyases are key enzymes involved in the degradation of pectin, a complex polysaccharide found in plant cell walls. This study explores the influence of sucrose, a common sugar, on the activity of microbial pectin esterases and pectate lyases. Enzyme assays were conducted using pectin as a substrate in the presence of varying concentrations of sucrose. The results indicated that sucrose exerted distinct effects on these enzymes, with some concentrations enhancing activity while others displayed inhibitory effects. The intricate interplay between sucrose concentration and enzyme activity provides insights into the regulation of pectin degradation pathways and underscores the complexity of microbial enzyme interactions.