DESIGN AND OPTIMIZATION OF EVEROLIMUS-LOADED PROTEIN NANOPARTICLES FOR TARGETED THERAPY OF GLIOBLASTOMAS

Аннотация

Glioblastoma is a highly aggressive and difficult-to-treat brain cancer with limited therapeutic options. This study aims to design and optimize protein nanoparticles loaded with Everolimus, a potent anticancer drug, for targeted therapy of glioblastomas. The protein nanoparticles were formulated using a biodegradable protein matrix and characterized for physicochemical properties, drug encapsulation efficiency, and drug release kinetics. Cellular uptake studies were conducted to assess the internalization of nanoparticles by glioblastoma cells. The results demonstrate uniform size distribution, stable surface charge, high drug loading efficiency, sustained drug release, and efficient cellular uptake of Everolimus-loaded protein nanoparticles. This research highlights the potential of protein nanoparticles as a promising strategy for improving the treatment outcomes of glioblastomas. KEYWORDS Glioblastoma, protein nanoparticles, Everolimus, targeted therapy, drug delivery, formulation optimization, sustained release, cellular uptake.