Особенности влияния гипертермии на экзокринную секреция поджелудочной железы растущих крыс

Аннотация

Daily effects on growing rats with a high external temperature led to phase changes in the activity of alpha-amylase in pancreatic tissue, the severity of which was different in animals receiving a particular diet. The task of the authors was to identify the specific features of the effect of hyperthermia on the activity of pancreatic enzymes. Key words: prepubertal, alpha-amylase, thermal exposures, high- carbohydrate, balanced, low-carbohydrate, juvenile, pancreas. Objective: T o identify the peculiarities of the effect of high temperature on the activity of enzymes of various stages of carbohydrate hydrolysis in rats of prepubertal age, who received a diet with an increased and reduced carbohydrate content. Specific activity of pancreatic alpha-amylase Daily effects on growing rats with a high external temperature led to phase changes in the activity of alpha-amylase in pancreatic tissue, the severity of which was different in animals receiving a particular diet. The activity of pancreatic alpha-amylase in rats receiving a balanced diet at the beginning of studies under the influence of high temperature was markedly reduced. On days 4 and 8, it was 38% and 55%, respectively, lower than in animals exposed to heat, but receiving the same diet. Later it sharply increased and on the 15th day of studies it was 4.5 times higher than the level of activity of the enzyme in control animals.By the end of the experimental period, the specific activity of the pancreatic alpha-amylase decreased markedly, but, nevertheless, it was 71% higher than the control level. Table 1 Specific activity of pancreatic alpha-amylase (mg / g protein) of juvenile rats in the dynamics of their 30-day diet on diets with different amounts of carbohydrates under conditions of thermal exposures Days of Groups of R A C I O N S experience animals Balanced Low-carb High-carb 0 Initial 62135± 2800 62135±2800 62135 ± 2800 4 64700 ± 2400 44200± 1300 175500±3300 Control An 40300 ± 5500 33400 ± 3500 66500 ± 12300 experience 8 Control 70900 ± 2500 41700 ± 1400 174000±4000 28 «M ODERN SCIENTIFIC CHALLENGES AND TRENDS» SCIENCECENTRUM.PL ISSUE 2 ISBN 978-83-949403-3-1 An 31700± 3800 30600 ± 3100 225400 ±18500 experience 15 53600 ±1100 4700 246200 ± 14500 Control An 70700 ± 2200 ± 3600 244000± 15900 experience 317300 ± 10700 30 12800± 1200 131000±5000 Control An 64300 ± 2600 11300±1500 39300 ± 2300 experience 109900 ± 1800 In animals kept on a low-carbohydrate diet, in the first days of exposure to high temperature there was an increase in alpha-amylolytic activity of the pancreas. On day 4 of the experiment, it was 21% higher than that of control animals. Subsequently, on day 8 of the studies, the activity of the enzyme in the organ of the experimental animals decreased noticeably and became 27% lower than in rats not exposed to a high external temperature. On the 15th and 30th days of the experiment, the levels of specific activity of alpha-amylase in the pancreas of the experimental and control rats did not differ. Certain changes in the activity of pancreatic alpha-amylase under the influence of a high external temperature were noted in rats kept on a carbohydrate- rich diet. On day 4, it was 62% lower than in control animals that did not undergo overheating, on the 8th day it increased and exceeded the level of activity in control animals by 30%, then by day 15 decreased to the control level and thereafter, by day 30 experiments, was 70% below control. Thus, the data suggest that a high external temperature has a marked effect on the activity of pancreatic alpha-amylase in juvenile rats contained on all diets. The first days of the experiment are characterized by a decrease in the specific activity of the enzyme in animals kept in a balanced and high-carbohydrate diet and a slight increase in its level in rats receiving a diet with a low content of polysaccharides.By the end of the studies, alpha-amylolytic activity in rats kept on a balanced diet increased under the influence of a high external temperature, and in animals that were on a high-carbohydrate diet is decreased. As a result of the fact that a high external temperature has a certain effect on the activity of pancreatic alpha-amylase, the reaction of the pancreas to the applied different food rations changes in animals undergoing hyperthermia. In Fig. the dynamics of alpha-amylase activity during 30 days of dietary application is presented, where the enzyme activity was taken as the initial (100%) on the first day.As can be seen from the figure, in rats receiving a balanced diet, the high external temperature on days 4 and 8, respectively, by 35% and 48% reduces the amylolytic activity of the pancreas. Then, on day 15 of the experiment, it sharply (more than 5 times as compared with the first day) increases, and by the end of the study it decreases, but not to the initial level: and on the 30th day, the activity of the enzyme is 77% higher than the level of the first day. On a low-carb d