Изучение стресса в аспекте физиологии

Аннотация

In our work, the stress reaction of the body, manifested in the digestive organs, was noted. Aggressiveness is an important form of zoosocial behavior in animals of different species, having adaptive significance and manifested by a species-specific set of behavioral reactions [4, 5]. But the peculiarities of the functioning of the digestive organs, depending on the behavioral characteristics of the body, are not sufficiently studied either in a normal state or under stress. Keywords: aspects, strength, organism, reaction. One of the directions considered stress as a behavioral response to socio-psychological stimuli. B.P. Dorenvendom modified G. Selye’s model of physiological stress. They approached stress as state of the body, which relies on adaptive and non-adaptive responses. Stressors are understood as social factors: economic or family failures, which act as objective events that disrupt (destroy) or threaten to undermine ordinary life individual. They are not necessarily negative and do not always lead to objective crisis. Important form of zoosocial behavior at animals of different types, having adaptive value and shown a species-specific set of behavioral reactions, is the aggression. It is known that digestive organs are usually involved in stress reaction of an organism. However, features of functioning of digestive organs depending on the behavioral characteristic of an organism are studied neither insufficiently normal nor at stress. Relevance of work was clarification of the functional condition of a small bowel at rats from nonaggressive group at a stress under the influence of Phenazepamum tranquilizer. Purpose of work: studying of influence of Phenazepamum on activity and a topography of enteral enzymes at rats from nonaggressive group in the conditions of an immobilized stress. Materials and methods. Experiments were made on adult not purebred rats with the body weight of 180-200 g. Three groups of rats - mixed (animals were not checked for aggression), nonaggressive and aggressive groups were used. Animals were checked for aggression by a technique A.L. Rylova (1983); an irritant were electric impulses, each of which was shown to animals quadruple. Aggression size, the bound to pain, was estimated on an index of "an average score of the fights" which arose in response to a series from 88 impulses and the number of fights from 88 possible. Aggressive rats are those who have "the average score of fights" from 45,6 to 39,7. At rats with average aggression this index fluctuates from 38,8 to 33,4. At nonaggressive individuals it makes 32,6-0. The immobilized stress was caused in rats by the forced immobilization within 24 clocks. Phenazepamumwas administeredorally with the preventive purpose in 30 minutes prior to a stress in a dose 2 mg/kg. As monitoring rats are used with the corresponding typological characteristic to whom orally entered the equivalent amount of distilled water. Weight mucous was determined by routine weighing. The activity of digestive enzymes was determined by the following techniques: monoglitseridlipaza - method of A.M. Ugolev and M.Yu. Chernyakhovskaya (1969), glycyl-1- Leucinum-dipeptidgidrolaza - method of A.M. Ugolev and N.M. Timofeeva (1969), amylase - © Askaryans V.P., Ergashov K.U. / Аскарьянц В.П., Эргашов К.У., 2022 35 ISSN 2311-2158. The Way of Science. 2022. № 11 (105). method Smith - the Swarm in A.M. Ugolev's modification (1969); saccharase - the Heleon method in modification of A.M. Ugolev and N.N. Iyezuitova (1969), lactase - the Dalhqvist (1968) method. The activity of enzymes was calculated on 1 g of mass of crude fabric of a mucosa of a small bowel and was expressed in mg/min/g for an amylase and in pmol/min/g for other enzymes. Statistical data processing was carried out by Student-Fisher's method. Results of researches. In this series of experiments as monitoring served intact rats from nonaggressive group. Nonaggressive ratsbore well a 24-hour immobilization. There were not lethal outcomes. The mass of a mucosa decreased in 6 h after an immobilization along all gut approximately by 1,5 times, in 24 h and further an index came back to monitoring level. In a homogenate of the mucosa removed along all small bowel, the activity of a monoglitseridlipaza was inhibited in 1,5; 2,2; 2,2 times in 6, 24, 48 h after a stress (tab. 1) The activity of a dipeptidgidrolaza increased through 6, 24, 48, h in 1,5; 1,6 and 2, 2 times. The activity of an amylase decreased throughout all experiment: in 6 h by 2,2 times, 24 h - by 2,4 times, 48 h - in the 1, 7 time. Thesaccharase activity was defined raised in 2, 3; 2,5; 2,7 times in 6, 24, 48 h after an immobilization. The activity of a lactase was inhibited by 2,2 times in 6 h, further did not differ from monitoring. Table 1 Activity of a monoglitseridlipaza (pmol/min/g) in a mucosa homogenate, removed along all small bowel at an immobilized stress and at a stress against the Phenazepamum at rats from nonaggressive gr