Quantitative analysis of microbiota in patients with orthopedic structures on dental implants using the real-time pcr method

Аннотация

Microbiological research was carried out by PCR method in 42 persons aged 25-60 years (men - 24 and women - 18), installed and Osseo integrated DIO dental implants, which were prototyped by metal-ceramic crowns, with temporary fixation on Tempo Bond NE. After 20 days, a follow-up visit was scheduled. All the examined patients were taken biological substrate of gingival fluid from the para-implant furrow using paper endodontic pins (№ 25). Exposure of two pins was made within 30 seconds with the help of tweezers between the peri-implant cuff and the crown, biological substrate of gum liquid was taken from the parai-implant furrow. The biological substrate was transported and tested according to the manufacturer's recommendations. When studying the content in patients with orthopedic constructions on dental implants by PCR method we observed high frequency of occurrence of Streptococcus sobrinus (69,3%), Streptococcus sanguis (48,6%) and Porphyromonas gingivalis (41,9). Relatively similar data on the content of these microorganisms were obtained in the molecular genetic study of Streptococcus oralis samples (22.7%). Obviously, it is the combined effect of the most frequently diagnosed pathogens and peculiarities of interaction of anaerobic agents of parasitocenosis that can determine the nature of inflammatory and destructive process in the peri-implant zone. Keywords: PCR, pathogenic, opportunistic pathogens, internal interface, paraimplant area, implant, abutment. INTRODUCTION composition in peri-implant is now known and Dental implantation continues to be one of the represents a large variety of aerobic and most important directions among the priority anaerobic microorganisms. The researchers problems of dentistry in the modern world. attach special importance to Prevotella Application of dental implants solves a intermedia, Porphyromonas gigngivalis, significant part of problems in case of partial Actinobacillum actinomycetam commitans, and complete absence of teeth, plays a decisive Bacteroides forsithus, Treponema denticola role in restoring the function of chewing, helps [8,10,11]. The inflammatory process of tissues to correct and improve the aesthetics of the tooth in the peri-implant zone is the main reason of row, smile and face in general. bone tissue destruction and resorption in the The experience of dental implants installation implant area. continues to accumulate in all its aspects [2,3]. The source of bacterial flora may be gaps and One of the conditions for the smooth hollow spaces in the inner surface (interface) of postoperative flow is the decrease of the the implant abutment, which will act as a microorganisms’ level in the implant bed area bacterial reservoir. Since usually used implants [1,4,9]. consist of two parts - the implant and the In spite of the fact that implantation in recent abutment - there is a connection place between years has a high level of success in the early them, which is the inner surface (interface) of the postoperative period, in the scientific literature implant. The technologically acceptable gap is there is more and more information about the from 2 to 5 μm, which is quite enough for risk of distant complications connected first of all penetration of typical representatives of with the development of inflammation of tissues pathogenic microflora of the oral cavity with surrounding the Osseo integrated implant comparable sizes - from 0.5 to 2.0 μm. [4,7,9,12]. The most likely cause of development in the OBJECTIVE OF THE STUDY tissues surrounding a functioning implant may To detect pathogenic microorganisms in patients be penetration of the oral infection into the area with orthopedic constructions on dental implants of implant-bone contact. The microbial by PCR method. 736| International Journal of Pharmaceutical Research | Apr - Jun 2020 | Vol 12 | Issue 2 Azim Olimov et al / Quantitative Analysis of Microbiota in Patients with Orthopedic Structures on Dental Implants using the Real – Time PCR Method RESEARCH MATERIAL AND METHODS Detection of the fluorescent signal is assigned In order to solve the stated goal we carried out through the channels for fluorophores FAM, microbiological research in 42 people aged 25- JOE, ROX and Cy5. If other tests are performed 60 years (men - 24 and women - 18), who were at the same time, detection is also assigned to undergoing treatment at the Department of other used channels. Surgical Dentistry and dental implantology of Tubes are placed in the cells of the reaction TGSI. This system has an implant-abutment module of the device and start the amplification connection by the type of hexagonal connection program with fluorescent signal detection. using a fixation screw. All patients had On the basis of the obtained threshold cycle (ST) orthopedic constructions (metal-ceramic crowns, values and on the basis of the given with temporary fixation on Tempo Bond NE) concentration values for DNA calibrators K1 and installed. After