SEPARATION OF ENDOPHYTIC BACTERIA ISOLATES FROM THE MEDICINAL PLANT INULA HELENIUM AND STUDY OF THEIR ENZYME SYNTHESIS PROPERTIES.

Аннотация

Isolated from the medicinal plant Kalonchoye degremona and molecularly identified Bacillus amyloliquefaciens - UzMU 22 local bacterial strain Verticillium dahliae, Alternaria alternata, Fusarium solani, Fusarium oxysporum, Candida albicans pathogenic fungi Candida albicans have high antagonistic properties. Among pathogenic bacteria, such as Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa, the strain Bacillus subtilis showed stronger antagonistic properties compared to other pathogenic bacteria. Keywords. Kalanchoe degremon, medicinal plant, pathogen, identification, strain, antagonistic property, antifungal, fungal, antibacterial, bacterial, Bacillus amyloliquefaciens. Log in. It is known that today biological preparations based on live cultures of endophytic bacteria that synthesize bioactive secondary metabolites are widely used in the biological protection of agriculture, as well as in obtaining various hydrolytic enzyme preparations and probiotics [1-3]. Especially important are antibiotics, improper nutrition, chemical preparations that inhibit the protection of normal intestinal flora from pathogenic bacteria and fungi causing various diseases, participate in the synthesis of several vitamins belonging to the vitamin K and vitamin B groups, and probiotic preparations containing living bacteria that are beneficial for the body and are used to prevent the development of dysbacteriosis due to the weakening of the organism due to various infections and the multiplication of conditionally pathogenic microbes. Currently, endophytic microorganisms synthesizing secondary metabolites can be isolated from many medicinal plants and widely used in agriculture, pharmaceuticals, and medicine [3-5]. In our previous studies, a total of 45 bacterial isolates were isolated from the internal tissues of the roots, stems, and leaves of medicinal plants found in field and household conditions, such as 14 ACADEMIC RESEARCH IN MODERN SCIENCE International scientific-online conference plantain, chamomile, chamomile, chicory, kalanchoe, lemon balm, and mint, which are included in the pharmacopoeial list. The degree of affinity of isolated endophytic bacterial isolates for 1% casein and 1% starch, as well as their cultural and morphological properties, such as hydrolysis zones, color, transparency, edges, and surface areas of colonies, were studied. Objective of the work: To study the antifungal and antibacterial properties of the bacterial strain Bacillus amyloliquefaciens - UzMU 22. Research and methods. Anti-fungal activity of the Bacillus amyloliquefaciens -UzMU 22 strain against some pathogenic fungi and antibacterial activity against pathogenic bacteria was revealed. Received results and their analysis. It is known that the possibility of isolating endophytic bacteria that synthesize secondary metabolites from medicinal plants and determining their antagonistic properties against phytopathogenic fungi and sensitivity to various antibiotics, as well as creating new generations of biological preparations based on mono- or mixed culture associations and their wide use in agriculture, is being implemented [1-4]. This study, the study of antifungal and antibacterial properties against various pathogenic fungi and bacteria that enter the body, is one of the urgent tasks from a scientific and practical point of view. Accordingly, the anti-fungal properties of the strain KB.amyloliquefaciens - UzMU 22 , isolated from alanchoye degremon, Verticillium dahliae, Candida albicans, Alternaria alternata, Fusarium solani and Fusarium oxysporum against pathogens, as well as against pathogenic bacteria Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa, were studied. B.amyloliquefaciens - UzMU 22 inhibition zone of the bacterial strain against various pathogenic fungi and bacteria Fusarium Fusarium Verticillium Alternaria Strain oxysporum solani dahliae alternata 6.8±0.4 8.2±0.38 8.4±0.41* 10.2±0.46* Candida Escherichia Bacillus Pseudomonas B.amyloliquefaciens - albicans coli subtilis aeruginosa UzNU 22 13.5±0.64* 8.2±0.34 16.4±0.62* 9.6±0.27 As can be seen from the table, the strain KB.amyloliquefaciens - UzMU 22 extracted from alanchoye degremon a series Verticillium dahliae, Alternaria alternata, Fusarium solani and Fusarium oxysporumhas a strong antifungal ability against the fungus Candida albicans, and from pathogenic bacteria Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa, the strain 15 ACADEMIC RESEARCH IN MODERN SCIENCE International scientific-online conference Bacillus subtilis has stronger antagonistic properties than other pathogenic bacteria. The obtained results show that this strain can be used as a probiotic in animal husbandry, as well as a non-pathogenic environmentally friendly strain in the food and pharmaceutical industries. Today, biological preparations consisting of anti-inflammatory and immunostimulating biologically active additives from medicinal plants growing in our