MODERN AND EFFECTIVE APPROACHES TO THE TREATMENT OF CANINE WOUNDS

Аннотация

This article presents the results of a study on the treatment of purulent wounds in dogs using the probiotic Innoprovet2 in various combinations (with and without antibiotic). It was found that under the influence of Innoprovet2 the average period of wound healing in dogs was: in the first experimental group 22-24 days, in the second experimental group 16-18 days, and in the control group - 26-28 days. During the experiment, the wound healing process and the condition of the dogs were observed for 28 days. ImitoMeasure program was used to evaluate the wound surface in all groups, and a systematic evaluation of the wound condition was performed. Keywords:Wound,probiotic,ImitoMeasure, antibiotic, ciprofloxacin, pus,sorbents, infection. Introduction At present, in both human and veterinary medicine, surgical disorders account for approximately 30–35% of cases, frequently manifesting as acute and chronic purulent diseases. The lack of comprehensive treatment strategies for wound processes disrupts homeostasis and impairs the function of internal organs, which in turn diminishes the efficacy ofpharmacological interventions and contributes to thedevelopment of complications [3]. A wide range of therapeutic agents—including antibiotics, proteolytic enzymes, bioactive sorbents, and physical treatment modalities such as laser therapy, ultrasound, and hyperbaric oxygenation—are routinely employed in the management of wounds and wound- related infections [2]. However, due to the limited scope of these therapeutic approaches, the treatment of purulent wounds remains a pressing challenge. Therefore, the development of novel therapeutic strategies and the implementation of environmentally safe agents constitute a majorfocus of contemporary research efforts [5,7,8,9]. Probiotic preparations can be administered in various formulations, including powders, pastes, or aerosols, often combined with auxiliary substances or sorbent mixtures [5]. A significant proportion of pathogenic microorganisms implicated in wound infections exhibit resistance to conventional antibiotics. Among the promising candidates with both antimicrobial and regenerative properties is the probiotic bacterium Bacillus subtilis, which is capable of synthesizingantibiotics [1,4]and bacteriocins [5]. These bacteria also enhance wound healing by producing proteolytic enzymes, demonstrating antioxidant activity [1,4,5,6], exerting thrombolytic effects, and preventing excessive scar formation. Several authors have reported the successful application of probiotic page16 INTERNATIONAL JOURNAL OF ARTIFICIAL INTELLIGENCE ISSN:2692-5206,ImpactFactor:12,23 AmericanAcademicpublishers,volume05,issue07,2025 Journal: https://www.academicpublishers.org/journals/index.php/ijai formulations such as Bioseptin, Bactisporin, Sporobacterin, and Bactisporin for the treatment of uninfected wounds, purulent-necroticprocesses, burns, and dermatitis. Objective of the Study This research was dedicated to investigating the effects of probioticadministrationin thetreatment ofpurulent wounds in dogs. Object of the Study Nine dogs were selected as experimental subjects and randomly allocated into three groups: two experimental groups and one control group. In the experimental animals, standardized wounds were created inthe femoral region usingthemethod described by Sukhovei(2008). Study Site The experiments were conducted at the Veterinary Surgery and Obstetrics Department Clinic of Samarkand State University of Veterinary Medicine, Animal Husbandry andBiotechnologies. Materials and Methods To assess the therapeutic properties of the probiotic Inoprovet- 2, the nine dogs were divided into three groups. In all animals, wounds were surgically induced on the lateral surface of the thigh and inoculated with Streptococcus pyogenes isolated in previous studies toestablish a model ofpurulent infection. After 72 hours, upon confirmation of purulence, all groups received daily wound irrigation witha 1:5000furacilin solution. In Experimental Group I (three dogs), drainage with Inoprovet-2 probiotic was applied to the wounds, and the probiotic was also administered orally at a dose of 5 ml of suspension (containing 1×10⁹ CFU/ml) twicedaily. In Experimental Group II, the probiotic was given orally at the same dosage, and ciprofloxacin was administered intramuscularly at a doseof 1ml. In the Control Group (Group III), wounds were treated topically with 0.4 ml of ciprofloxacin solution. For oral administration, the dry probiotic was reconstituted at a concentration of 1 g per 1,000 ml of water. For topical application (drainage), it was diluted at a ratio of 1 g per 100 ml ofphysiological saline(tab-1). Table1. Experimental design forassessing theefficacy of Inoprovet-2 probioticin dogswith purulentwounds Number Group TreatmentModality Dosage andApplicationDetails ofDogs Oral: 5 ml of suspension (1×10⁹ Experimental Inoprovet-2 probiotic CFU/ml) twice daily; Topical: 3 Group I (oral + topical drainage