Technology for Obtaining the Biologically Active Supplement “Cardioherb”

Аннотация

Currently, cardiovascular diseases are the leading cause of death and disability among humans [1,2]. In this regard, the development of herbal medicines not only for treatment but also for prevention is considered highly relevant. Dietary supplements (biologically active food additives or BAFAs) are mainly used as preventive agents [3]. Keywords: Biologically active, preventive agents, scientific research. technology for obtaining extracts from hawthorn fruits Introduction: It is well known that for a long time, and Geranium collinum roots and rhizomes, and on this plant-based medications such as hawthorn and basis, to produce a dietary supplement in capsule form Geranium collinum have proven to be effective named “Cardioherb.” cardioprotective agents [4]. These plants have been widely used not only in folk medicine but also in Research Results. During the development of scientific research. Numerous studies indicate that extraction technology, several factors were considered: these plants are rich in various biologically active degree of raw material grinding, type of extractant, compounds, making them highly valuable in medicine. extraction method, chemical composition, etc. Research Objects. The research utilized hawthorn Hawthorn fruits were ground into sizes of 1–2 mm, 3– fruits (Crataegus) and roots/rhizomes of Geranium 5 mm, and 7–8 mm. Extracts were obtained using collinum, which grow in the Republic of Uzbekistan. maceration, repercolation, and the method of VNIIF (All-Union Scientific Research Institute of Pharmacy). Research Objective. The literature contains data on The content of flavonoids—dihydroquercetin, luteolin, obtaining extracts from hawthorn fruits and the rutin, rosavin, quercetin, salidroside—was determined underground parts of Geranium collinum [5]. However, in the extracts using HPLC (High Performance Liquid despite numerous studies, no combined Chromatography) (see Figure 1). phytopreparation from these two plants currently exists. The goal of our research is to develop a American Journal Of Biomedical Science & Pharmaceutical Innovation 19 https://theusajournals.com/index.php/ajbspi American Journal of Applied Science and Technology (ISSN: 2771-2745) Figure 1. Flavonoid content in hawthorn extracts Ethanol at different concentrations (30%, 50%, 70%) was used as the extractant (Tables 1– 3). Table 1. Content of extractive substances in hawthorn extract (maceration method) Article size Flavonoids Ethanol concentration, % (mm) 30 50 70 Flavonoid content mg/g Dihydroquercetin 0,012 0,017 0,015 Luteolin 0,0098 0,012 0,010 Rutin 0,037 0,041 0,035 1-2 Rosavin 0,113 0,123 0,121 Quercetin 0,0095 0,011 0,010 Salidroside 1,62 1,65 1,58 Dihydroquercetin 0,017 0,018 0,0186 Luteolin 0,012 0,013 0,0131 3-5 Rutin 0,041 0,045 0,0453 Rosavin 0,123 0,125 0,126 Quercetin 0,011 0,013 0,0137 Salidroside 1,65 1,68 1,68 Dihydroquercetin 0,016 0,015 0,012 Luteolin 0,011 0,010 0,0098 7-8 Rutin 0,039 0,035 0,037 Rosavin 0,122 0,121 0,113 Quercetin 0,011 0,010 0,0095 Salidroside 1,62 1,58 1,62 American Journal of Applied Science and Technology 20 https://theusajournals.com/index.php/ajast American Journal of Applied Science and Technology (ISSN: 2771-2745) Table 2. Content of extractive substances in hawthorn extract (repercolation method) Flavonoids Ethanol concentration, % Article size 30 50 70 (mm) Flavonoid content mg/g Dihydroquercetin 0,012 0,017 0,015 Luteolin 0,0098 0,011 0,010 Rutin 0,037 0,041 0,032 1-2 Rosavin 0,112 0,123 0,121 Quercetin 0,0095 0,011 0,010 Salidroside 1,62 1,65 1,59 Dihydroquercetin 0,017 0,018 0,018 Luteolin 0,012 0,013 0,013 3-5 Rutin 0,041 0,043 0,049 Rosavin 0,121 0,125 0,126 Quercetin 0,011 0,013 0,013 Salidroside 1,65 1,69 1,69 Dihydroquercetin 0,016 0,015 0,012 Luteolin 0,011 0,010 0,0098 7-8 Rutin 0,038 0,035 0,036 Rosavin 0,112 0,121 0,113 Quercetin 0,011 0,010 0,0093 Salidroside 1,62 1,58 1,54 Table 3. Content of extractive substances in hawthorn extract (VNIIF method) Flavonoids Ethanol concentration, % Аrticle size 30 50 70 (mm) Flavonoid content mg/g Dihydroquercetin 0,015 0,016 0,008 Luteolin 0,012 0,011 0,0005 Rutin 0,041 0,03 0,016 1-2 Rosavin 0,113 0,11 0,095 Quercetin 0,011 0,012 0,0093 Salidroside 1,65 1,71 1,43 Dihydroquercetin 0,018 0,02 0,009 Luteolin 0,013 0,014 0,0005 3-5 Rutin 0,045 0,05 0,02 Rosavin 0,125 0,13 0,097 Quercetin 0,013 0,014 0,0095 Salidroside 1,68 1,77 1,45 American Journal of Applied Science and Technology 21 https://theusajournals.com/index.php/ajast American Journal of Applied Science and Technology (ISSN: 2771-2745) Dihydroquercetin 0,013 0,014 0,008 Luteolin 0,011 0,009 0,0005 7-8 Rutin 0,038 0,02 0,02 Rosavin 0,098 0,11 0,094 Quercetin 0,087 0,011 0,0093 Salidroside 1,58 1,69 1,38 Tables 1 to 3 show the concentration of flavonoids in The second stage of the study focused on developing a hawthorn extracts obtained via maceration, technology for producing a dry extract from Geranium repercolation, and VNIIF methods, respectively. collin