Heterologous expression of membrane proteins

Аннотация

This volume proposes an overview of different heterologous expression systems that produce an adequate amount of membrane proteins for structural analysis. Methods and protocols are described for each heterologous expression system proposed.Some chapters of this volume treat membrane protein solubilization, purification and instability in solution and comment on the strategies that allowed the determination of the structure of the first heterologously expressed mammalian membrane proteins.