DETERMINATION OF PROTEIN AND AMINO ACID COMPOSITION OF THE DEVELOPED DRY EXTRACT "IMMUNASHIP"

Аннотация

Herbal preparations that enhance immunity, due to the presence of a variety of biologically active substances in them, gently affect the body and restore impaired functions of the immune response. For the prevention of immune system disorders, preparations based on purpleechinacea (Echinacea purpurea ),as they have apositiveeffect onthe body's immunityto infectious agents and reduce the manifestations of allergic diseases. This article defines the protein and amino acid composition of the dry extract "Immunaship". The protein in the dry extract " Immunaship " was determined by the Kjeldahl method for the quantitative content of nitrogen. The amino acid composition of thedry extract was studied and 20 free amino acids were identified, with a quantitative content of 8.14969 mg/g, 10 of which are essential, which indicates thebiological valueof thedry extract " Imunaship " based onpurpleechinacea herb and rosehips. Key words: Protein, amino acid composition, dry extract, Immunaship, HPLC, Kjeldahl method, purpleechinacea, rose hip, immunity. Introduction. Understanding the role of drugs that enhance immunity in the biosystems of a living organism is the key to creating new classes of effective dosage forms. The use of environmentally friendly plant materials is based on the deep relationship of natural components with the human body [1,2,3]. The healing properties of the plant are due to the action of a complex of biologically active substances contained in the plant, their harmonious interaction and optimal ratio when acting on the human body. Research into herbal medicines with the aim of introducing them into medical practice, developing optimal technologies for obtaining dry extract and studying the biologically active substances in them that determine their pharmacological action is very relevant[4,5,6,7]. Herbal preparations that enhance immunity, due to the presence of a variety of biologically active substances in them, gently affect the body and restore impaired functions of the immune response. To prevent immune system disorders, preparations based on purple echinacea (Echinacea purpurea), as they have a positive effect on the body's immunity to pathogens of infectious diseases and reduce themanifestations ofallergic diseases,[8,9,10,11,12]. The effectiveness of purple echinacea can be enhanced by combining it with medicinal plants with antioxidant action, as well as byoptimizing thedosage form [13,14,15,16,17]. 146 INTERNATIONAL MULTIDISCIPLINARY JOURNAL FOR RESEARCH & DEVELOPMENT SJIF2019:5.2222020:5.5522021:5.6372022:5.4792023:6.5632024:7,805 eISSN:2394-6334 https://www.ijmrd.in/index.php/imjrd Volume12,issue06(2025) Purpose of the study Determination of protein and amino acid composition of the developed dry extract " Immunoship " based onpurpleechinacea herb and rose hips. Materials and methods The object of the study was the extract from the soup “Immuna ship” based ontheherb Echinacea purpurea and rose hips. Identification of amino acid derivatives in the dry extract was carried out by HPLC using an Agilent chromatograph. Technologies 1200 with DAD detector, with 75 x 4.6 mm column Discovery H S C . The mobile phase consists of solution (A): 0.14 M sodium acetate and 0.05% 18 triethylamino, at a solution pH of 6.4; Solution (B) consists of acetonitrile. Chromatographic analysis was carried out in the following mode: I - 1-6% / 0-2.5 min; II - 6-30% / 2.51-40 min; III - 30-60% / 40.1-45 min; IV - 60-60% / 45.1-50 min; V - 60-0% / 50.1-55 min, flow rate 1.2 ml/min, at UVdetector absorption of269nm,at room temperature. Protein was determined bytheKjeldahl method. Experimental part In plants, amino acids are found in a free and protein-bound state, and during extraction, both free and bound amino acids pass into the extract. When determining free amino acids in a dry extract, protein-bound amino acids and peptides interfering with the analysis were precipitated by centrifugation. For this, an exact volume of 1 ml of 20% trichloroacetic acid was added to 1 ml of the sample being studied . The sediment was precipitated in centrifuge cups for 10 min at a rotation speed of 8000 rpm. 0.1 ml was collected from the supernatant liquid and dried in an air- lyophilic dryer. The hydrolyzate was evaporated, the dry residue was dissolved in a mixture consisting of triethylamine - acetonitrile - water in a ratio of (1:7:1) and dried. In order to neutralize the acids, this procedure was repeated twice. Phenylthiocarbamyl derivatives (PTC) of amino acids were obtained with a reaction with phenylthioisocyanate according to the Steven method. A ., Cohen Daviel . The obtained data on the composition and quantitative content of amino acids are given in Table 1, the chromatograms of the standard sample and dry extract are shownin Fig .1and Fig .2,respectively.[18,19,20,21,22] Table 1 Compositionof free amino acids inthedry extract " Immunaship" No. Name ofamino acids Content,mg/g 1 Aspartic acid 0.083098 2 Glutamicaci