Positive Effect of Pseudomonas Chlororaphis On Mung Bean Germination

Аннотация

In this article, we can see that the bacterium Pseudomonas chlororaphis had a very positive effect on germination when exposed to two different varieties of mung bean plants using two different dilutions of this strain. We observed that the most effective results were obtained from the dilutions of the bacterial strain at 1:19 and 1:49, with the 1:19 dilution being the most effective. Keywords: Fusarium solani, Aspergillus ochraceus, Fusarium culmorum, Pseudomonas chlororaphis, Cladosporium sp., Aspergillus flavus, Fusarium verticillioides, Alternaria tenuissima, Navro'z, Kahrabo, Inhibition zone, phytopathogenic fungi. Introduction: Phytopathogenic fungi cause significant damage to plants. Phytopathogenic fungi are a serious problem in agriculture, negatively affecting crop quality and productivity [1]. The activity of phytopathogenic fungi in plants is difficult to control, causing significant losses for many crops [2]. Bacterial strains that combat such phytopathogenic fungi and stimulate plant growth are very useful. On this basis, we aimed to observe the positive effect of the bacterium Pseudomonas chlororaphis on the vegetative period of mung bean plants and the high antagonistic activity of this bacterial strain against phytopathogenic fungi such as Fusarium European International Journal of Pedagogics 231 https://eipublication.com/index.php/eijp European International Journal of Pedagogics solani, Aspergillus ochraceus, and Fusarium showed a high level of antagonistic activity against these culmorum[3]. phytopathogens. When seeds from two different varieties of mung bean were exposed to a liquid-based The research used a strain of the bacterium method, germination was significantly affected Pseudomonas chlororaphis from the collection of the compared to the control. Two different varieties of this Microbiology Laboratory and phytopathogenic fungi mung bean plant are widely cultivated in our country. such as Fusarium solani, Aspergillus ochraceus, and This had a positive effect on both varieties, and it was Fusarium culmorum. Two widely cultivated varieties of especially effective on the Navruz variety. When seeds mung bean, Navruz and Qahrab, were used in were treated with a liquefied solution of this bacterial agriculture. In laboratory conditions, phytopathogenic strain, we can see that the 1:19 ratio produced the most fungi and bacterial strains were grown on Mpa and effective results when the liquefied solution was Chapek nutrient media. Antagonistic activity was prepared in two different ratios, 1:19 and 1:49. We can assessed using the well-diffusion method. Agar see that there was a significant difference in the medium was placed in Petri dishes, phytopathogenic germination of mung bean plants when treated with a fungi were sown using the lawn method, and bacterial dilution of the bacterial strain compared to the control. culture fluid was placed in the well. Incubation was (figure 2.) continued for 3-7 days at 30°C, and the affected zones were measured in millimeters, and the bacterial strain European International Journal of Pedagogics 232 https://eipublication.com/index.php/eijp European International Journal of Pedagogics Figure 2. Effect of bacterial strain on mung bean germination Fusarium solani, Aspergillus ochraceus, and Fusarium We can see that the germination of the mung bean culmorum. It had moderate antagonistic activity against plant was positively affected by the dilution of the Penicillium sp., Fusarium oxysporium, and Alternaria bacterial solution at a ratio of 1:19. When seed alternata, and no significant antagonistic activity was germination was monitored compared to the control, observed against Cladosporium sp., Aspergillus flavus, we observed that 1:19 and 1:49 were also very Fusarium verticillioides, and Alternaria tenuissima. effective. According to the results, the P. chlororaphis strain formed inhibition zones of 28–34 mm against Figure 1. Effect of Pseudomonas chlororaphis on phytopathogenic fungi Phytopathogenic fungi Diameter of the inhibition zone, mm Fusarium solani 34 Aspergillus orchaceus 29 Fusarium culmorium 28 Penicillium sp. 15 Fusarium oxysporium 14 Alternaria alternata 13 Cladosporium sp. - Aspergillus flavus - Fusarium verticillioides - Alternaria tenuissima - table 1. Pseudomonas chlororaphis strain has high antagonistic The result observed when the zone of inhibition of activity against some phytopathogenic fungi. When phytopathogenic fungi under the influence of the applied to mung bean plants, seed germination rates bacterium Pseudomonas chlororaphis was measured increased. This bacterium is considered promising as a in diametric mm. In an experiment conducted on the biological control agent and as a biofertilizer. Navruz and Qahrab varieties of mung bean, dilutions of P. chlororaphis strain at 1:19 and 1:49 had a positive REFERENCES effect on seed germination. In particular, the Navruz Nizametdinova Ya.F., Mansurova M.L., Muzaffarova I.A., variety p