The Prognostic Value of Markers of Bone Metabolism

Аннотация

Biomarkers of bone metabolism, including biomarkers of bone collagen, have attracted great scientific interest. Numerous international studies indicate the importance of these markers in assessing the degree of bone tissue damage, which have not only diagnostic but also prognostic value. Keywords: Biomarkers of bone metabolism, bone tissue, acute hematogenous osteomyelitis, collagen. The study was conducted at the Samarkand State Medical University, a children's multidisciplinary Introduction: Today, acute hematogenous surgical hospital. For the osteomyelitis (CGO) remains a serious problem in childhood. According to WHO, the annual incidence of acute respiratory viral infections among children study, we took 60 sick children, who were divided into ranges from 2 to 13 cases per 100,000 population, 6 groups. Group 1 included sick children aged 0-28 depending on the region, age and quality of medical days, group 2: 1-11 months, group 3: 1-4 years, group care. In developed countries such as the USA, Canada 4: 5-9 years, group 5: 10-14 years, and group 6: 15-19 and Western European countries, the incidence of years. Children from the control group were assigned osteomyelitis in children remains relatively stable – to each study group. Concentrations of type I about 5-7 cases per 100,000 children per year. procollagen aminotherminal propeptide (PINP) and However, in Africa, South Asia, and Latin America, the type I collagen carboxytherminal telopeptide (β- incidence of CSOs is significantly higher, reaching 15-20 CrossLabs), as well as levels of calcium, phosphorus, cases per 100,000 children. In addition, numerous and alkaline phosphatase were studied in all children. studies indicate an increase in the incidence of These indicators were determined on the 1st day of osteomyelitis, which is associated with the growth of admission, on the 7th day after surgical treatment, and antibiotic–resistant bacterial strains (for example, in the long-term period after treatment (after 6 MRSA - methicillin-resistant Staphylococcus aureus), as months). To study these indicators, methods of enzyme well as insufficient vaccination against hemolytic immunoassay and biochemical analysis were carried streptococcus and pneumococcus, a high incidence of out. injuries among children, limited access to medical care RESULTS AND DISCUSSION and untimely treatment of bacterial infections, high survival newborn children with immunodeficiency due Bone tissue is a highly specialized metabolically active to the development of neonatology. mineralized connective tissue. Its structural and functional integrity is ensured by two multidirectional The purpose of the study processes, such as the formation and resorption of In this regard, the purpose of our study was to study bone tissue, the intensity of which is determined by the biomarkers of bone metabolism, namely type I activity of the cellular elements of bone, that is, procollagen aminotherminal propeptide (PINP) and osteoblasts, osteoclasts and osteocytes. Osteoblasts type I collagen carboxytherminal telopeptide (β- are responsible for formation, osteoclasts for CrossLabs) in children with acute hematogenous resorption, osteocytes for maintaining the structural osteomyelitis. and functional activity of mature bone tissue. The METHODS most important biopolymer of bone tissue is protein, type 1 collagen, which makes up more than 90% of the International Journal of Medical Sciences And Clinical Research 88 https://theusajournals.com/index.php/ijmscr International Journal of Medical Sciences And Clinical Research (ISSN: 2771-2265) mass of the organic bone matrix, which plays a key role surgery, the content of -CrossLaps in blood serum in the mineralization of the intercellular matrix of bone increased by 119.3% compared with the control group tissue, as well as in ensuring the strength properties of and amounted to 2.446 ng/ml (P=0.001). The amount bone. of type I collagen formation marker in bone tissue increased significantly before surgery, on day 7 after Thus, the metabolic state of the main bone protein, surgery, and after 6 months of follow-up, respectively, collagen, is determined by the intensity and direction from 6.31 ng/ml (in the control) to 9.58 ng/ml of its synthetic or, conversely, catabolic processes. This (P=0.012), 10.25 ng/ml (P=0.012), and 15.77 ng/ml indicates that shifts in bone collagen metabolism (p=0.001), respectively. directly reflect the general trend of metabolic processes in bone tissue. The balance between the The results of the studied indicators in the 2nd group of synthesis and breakdown of bone collagen is a complex children (1-11 months) indicate an increase in both multi-stage process involving both extracellular and resorption markers and markers of type I collagen intracellular stages. The levels of bone formation or formation in the blood serum. Thus, the content of - bone breakdown can be assessed by analyzing the CrossLaps was increased both before sur