EXPERIMENTAL VALIDITY OF THE PERSPECTIVE OF THE USE OF CREATIN PHOSPHATE AS A COMPARISON DRUG IN STUDYING THE CARDIOPROTECTIVE ACTIVITY OF MEDICINAL SUBSTANCES

Аннотация

This article is devoted to evaluating the possibility of using creatine phosphate as a comparison drug in the study of cardioprotection of medicinal substances. The study was conducted on healthy male Wistar rats. Langendorff's method was chosen as an experimental model for recreating myocardial infarction. During the experiment, a rat was anesthetized, then a bilateral laparotomy was performed along the mid-muscular lines, turning into a thoracotomy, followed by extraction of the heart and hanging it on a cannula attached to the Langendorff apparatus. During the experiment, the heart was perfused with oxygenated Krebs-Henseleit solution at 37C, in parallel, myocardial contractility, automatism were evaluated, as well as samples were collected for biochemical and histological studies. As a result of the study, the acceptability of the use of creatine phosphate as a comparison drug in assessing the cardioprotective properties of drugs was revealed.